Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress In Veterinary Medicine》 2000-04
Add to Favorite Get Latest Update

Construction of the Thymidine Kinase Gene Deleted Pseudorabies Virus Transfer Vector Containing the ORF5 of Porcine Reproductive and Respiratory Syndrome Virus

QIU Hua ji, ZHOU Yan jun, KONG Ling da, ZHANG Shao jie, QIAN Ping, WANG Liu,TONG Guang zhi * (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS,Harbin 150001,China)  
Pseudorabies virus (PRV) genome DNA was extracted and digested by restriction endonuclease KpnI. The KpnI fragment J of 5.9 kb was shown to contain thymidine kinase (TK) gene by polymerase chain reaction (PCR), and it was cloned to pUC119, resulting a recombinant pBTK5.9. The restriction and PCR analyses confirmed the TK gene was located in its PstI KpnI 2.6 kb fragment. The minor fragment was then subcloned to produce recombinant pBTK2.6. Porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 under the control of CMV promotor was inserted into the pBTK2.6. The resulting transfer plasmid pBdTK CMV ORF5 will be used for devolping TK - and gE - deletd recombinant PRV expressing PRRSV ORF5.
【Key Words】: pseudorabies virus porcine reproductive and respiratory syndrome virus thymidine kinase gene transfer vector8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 FAN Weixing1,SONG Jianlan2,WEI Rong2,CHEN Puyan2,ZHAO Hongkun1 (1College of Veterinary Medicine,Shandong Agricultural University,Taian 271018,China; 2College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Construction of TK Gene-deleted PRV Bartha-K61 Strain Recombinant Expressing EGFP[J];Chinese Journal of Animal and Veterinary Sciences;2003-05
Chinese Journal Full-text Database 1 Hits
1 Ding Jianhua Wang Jiafu Luo Manlin Fu Zhenlie Zheng Youxian Lei Liang Zhang Chuyu  (Institute of Virology, Wuhan University, Wuhan 430072);PCR Amplification and Cloning of tk Gene of Pseudorabies Virus HS 9304 Strain[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1998-02
Chinese Journal Full-text Database 10 Hits
1 CHEN Jian-jun~(1,2),ZHANG Yu-jin~1,YANG Zheng-qi~2,SONG Chang-xu~1(1.Veterinary Medicine Institute, Guangdong Academy of AgriculturalSciences,Guangzhou,Guangdong,510640,China;2.College of Animal Science and Veterinary Medcine,Northwest Sci-Tech University of Agriculture and Forestry,YangLing,Shaanxi,712100,China);The Progress in Molecular Biology of Duck plague virus[J];Progress In Veterinary Medicine;2003-06
2 SHEN Zhi qiang,LI Qiang,PANG Wan yong,GUAN Yu,JIA Xing lin,ZHUANG Jin qiu (Shandong Binzhou Institute of Animal Science & Veterinary Medicine,Binzhou,Shandong,256618,China);Histopathologic Comparison on the Effect of PR Inactivated Propolis Vaccine and Oil Vaccine to the Injection Site of Mice[J];Progress In Veterinary Medicine;2004-01
3 LI Zhen-guang,WU Hua(Institute of Special Wild Economic Animal and Plant Science,CAAS,Jilin,Jilin,132109,China);Progress on Genomic and Genetic Variations of Porcine Reproductive and Respiratory Syndrome Virus[J];Progress in Veterinary Medicine;2009-02
4 WEI Hong,LIN Feng-qiang,ZHOU Lun-jiang,WANG Long-bo,ZHUANG Xiang-sheng (Institute of Veterinary and Husbandry,Fujian Academy of Agriculture Sciences,Fuzhou 350013,China);Cloning and Sequence Analysis of Porcine Reproductive and Respiratory Syndrome Virus (Fuzhou Strain) ORF2-7 Gene[J];China Animal Husbandry & Veterinary Medicine;2007-01
5 ZHU Liang-qiang et al(Anhui Center for Animal Disease Control and Prevention,Hefei,Anhui 230032);Investigation on the Clinical Immunology Effect of PRRS Vaccine in Anhui Province[J];Journal of Anhui Agricultural Sciences;2009-27
6 AI Wei-chang~1 JIA Rong-ling~2 AIU Hui~3 MENG Han-bing~4 (1.Henan province wildness animal rescue center,Henan Zhengzhou 450044;2.Henan province nayang ariculture college,Henan nayang 473006;3.Henan province meat cow center,Henan Zhengzhou 450003;4.Zhengzhou animal breeding bureau,Henan zhengzhou 450007);Analyzing The Antibody Of PRRSV And PRV With the method of ELISA[J];Journal of Zhengzhou College of Animal Husbandry Engineering;2008-01
7 LIU Sheng-bing,YANG Qian *,JIANG Ping (Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture, Nanjing Agricultural University,Nanjing 210095,China);Effect of the immunization with PRRS attenuated vaccine by genital tract on antibody-secreting cells in the sows′ local uterus[J];Journal of Nanjing Agricultural University;2005-01
8 ZHU Dao-yu(The Department of Life Sciences,Heze University,Heze 274015,Shandong,China);Effect of intraperitoneal injection of indigowoad root polysaccharide on antioxidant enzyme activity and MDA content in the liver of Pelodiscus sinensis[J];Journal of Shandong University(Natural Science);2009-07
9 JIANG Yun-Bo, FANG Liu-Rong, XIAO Shao-Bo, ZHANG Hui and CHEN Huan-Chun~*Laboratory of Animal Virology,College of Animal Science and Veterinary Medicine,Huazhong Agriculture University,Wuhan 430070,China;Construction and Immunogenicity of Recombinant Pseudorabies Virus Expressing the Modified GP5m Protein of Porcine Reproduction and Respiratory Syndrome Virus[J];Chinese Journal of Biotechnology;2005-06
10 QIAN Ping, LI Xiang min, CHEN Huan chun *, JIN Mei lin, HE Qi gai (Laboratory of Animal Virology, College of Animal Husbandry and Veterinary Sciences, Huazhong Agricultural University, Wuhan 430070, China);Construction of the Recombinant Pseudorabies Virus Expressing the GP3 Gene of Porcine Reproductive and Respiratory Syndrome Virus[J];Chinese Journal of Biochemistry and Molecular Biology;2003-03
Chinese Journal Full-text Database 10 Hits
1 Wang Qin; Guo Wanzhu; Lou Gaoming ;Yan qigui; Wang Mingshu; Yu Guanghai( Sichuan Agricultural University, Sichuan 625014 )Thymidine kinase (TK) deletion mutants (TK-) of Pseudorabies virus (PRV) Fa strain were constructed by molecular biological methods.;CONSTRUCTION OF THYMIDINE KINASE DELETION MUTANTS OF PSEUDORABIES VIRUS FA STRAIN[J];Chinese Journal of Virology;1996-04
2 WANG Jia-fu 1,ZHANG Chu yu 1,DING Jian hua 1 ,ZHOU Rong 2 WEN Shu juan 2 HUANG Zheng hua 2 (1. Institute of Virology, Wuhan University, Wuhan 430072 China; 2. Gene Reserch Lab ,Nanfang Hospital, Guangzhou 510515, China);Cloning and Sequencing of gD Gene of Pseudorabies Virus of Hubei Strain[J];CHINESE JOURNAL OF VIROLOGY;2000-01
3 CHEN Huan-chun, ZHOU Fu-chun, FANG Liu-rong, HE Qi-gai, WU Bin, HONG Wen-zhou (Lab of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China);Construction of TK~-/gG~-/LacZ~+ Mutant of Pseudorabies Virus Strain Ea[J];Chinese Journal of Virology;2001-01
4 Chen Dehui,Zhang Heqiu,Wang Guohua(Institute of Basic Medical Sciences,Beijing,100850,China));Application of Electron Microscopy to the Diagnosis of Viral Infections[J];Journal of Chinese Electron Microscopy Society;1997-05
5 Wei Zhenming1; Wu Ping1;Chen Lipu2;Dai Chaojie1 and Li Yiying1(1Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences,Fuzhou 350013 ;2Fujian Biolokical Medicine Factory,Fuzhou 350014);Biological Characteristics of Pseudorabies Virus(PRV)Vaccine Strain[J];;1995-04
6 Fang Liurong Chen Huanchun Xiao Shaobo Wang Gefei Ma Xiangru (Huazhong Agricultural University, Wuhan 430070, China);Construction of a Universal Transfer Vector Deleting gG of Pseudorabies Virus[J];Journal of Huazhong Agricultural;2001-04
7 Xu Zhiwen Guo Wanzhu et al (Animal technical college of Sichuan Agriculture University,Yaan Sichuan 625014);Isolation and Identification of Pseudorabies Virus Strain SN and SL[J];SICHUAN ANIMAL AND VETERIN SCIENCES;2000-03
8 WANG Li-Xia WANG Yong HU Yuan-Lei GAO Yin LIN Zhong-Ping * (National Key Laboratory of Protein Engineering and Plant Molecular Biology of Peking University,Beijing 100871, China);Progress in the Study of the Structure and Function of Cre Recombinase[J];Chinese Journal of Biotechnology;2002-05
9 Wang Qian\ Wang Bin (Insititute of Genetics, Chinese Academy of Sciences, Beijing 100101);Progress in Bacterial Artificial Chromosome Research[J];Progress In Biotechnology;2002-03
10 Qu Yanchun Deng Changyan Liu Jianzhong Liu Guilan (College of Animal Science and Veterinary, Huazhong Agricultural University, Wuhan 430070);Recent Progress in Research on Gene Recombination Mediated bycre/lox P System[J];Biotechnology Information;2001-05
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 SU Xin-ming,YU Chun-mei,WANG Min-xiu,CHEN Yong-jun,CAO Rui-bing,ZHOU Bin,CHEN Pu-yan~*(Key Laboratory of Animal Disease Diagnostic and Immunology,Ministry of Agriculture,College of Veterinary Medicine,Nanjing Agriculture University,Nanjing 210095,China);Cre/LoxP Mediated Report Gene Deletion from Pseudorabies Virus[J];Chinese Journal of Virology;2006-06
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Luo Manlin; Ding Jianhua; Wang Jiafu; Zhang Chuyu(Department of Virology,Wuhan University Wuhan 430072);Amplifiction and Cloning of Protein Kinase Gene of Pseudorabies Virus by PCR[J];VIROLOGICA SINICA;1996-04
2 Guo Baoqing(Harbin Veterinary Research Institute,the Chinese Academy of Agricultural Sciences);Isolation and Identification of Porcine Reproductory and Respiratory Syndrome(PRRS) Virus from aborted fetuses suspected of PRRS[J];;1996-02
3 Lou Gaoming (Bioeng Lab of Agri and Anim Sci, Changchun 130062) Lin Shaorong Huang Yumao Su Danping (Fac of Vet Med,South China Agri Uni);An Outbreak of Porcine Reproductive and Respiratory Syndrome in Piglets[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1998-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved