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《Progress In Veterinary Medicine》 2003-01
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The Molecular Basis for the Antigenic Diversity of Type 1 Pili on Avian Escherichia coli

GAO Song,PENG Daxin,WU Xiaodong,ZHANG Rukuan,LIU Xiufan(Department of Veterinary Science, Yangzhou University,Yangzhou,Jiangsu,225009,China)  
In order to determine the molecular basis for the antigenic diversity of type 1 pili on avian Escherichia coli, the genomic DNA samples of avian Escherichia coli isolates 037(O78), 166(O78), 120(O18) and porcine Escherichia coli isolate 107/86 were extracted respectively, and used as templates for polymerase chain reaction (PCR) to amplify the pilA gene encoding the major subunit protein of type 1 pili. The specific amplicons about 570bp in length were cloned into T vector, and 4 positive recombinant plasmids were obtained by RE digestion analysis.The nucleotide sequences of pilA genes from 4 Escherichia coli isolates were determined. By comparison, the homology of amino acid sequences of FimA proteins encoded by pilA of those isolates was 94.3% to 99.0% among 3 avian isolates, and 89.6% to 91.1% between avian isolate and porcine isolate. These results suggested that antigenic diversities of type 1 pili exist not only between avian Escherichia coli isolates and procine Escherichia coli isolate,but also exist between different avian Escherichia coli isolates beloneged to the same O serotype, e.g.isolate 037 O78 isolate and 166 O78.
【Fund】: 国家自然科学基金资助项目(39770562)
【CateGory Index】: S852.6
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