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《Progress In Veterinary Medicine》 2004-06
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Microsatellite DNA Markers And its Application in Genetic Detection of Inbred Line Swine

HUO Jin-long,ZHANG Juan,LUO Gu-yue,ZHANG Mei,PAN Wei-rong,ZENG Yang-zhi (Banna Mini-pig Inbred Lines Key Laboratory of Yunnan Province,Kunming,Yunnan,650201,China)  
DNA microsatellite markers in nucleic genome of domestic animals were first discussed briefly, including conception, structure, distribution, composition, conservation, advantages and rich polymorphism of microsatellite and so on. And application of microsatellite marker technique in genetic detection of inbred line swine was clarified in detail.Prior to appearance of microsatellite-marker technique, inbred cofficient only can be simply calculated by formula;but now, with microsatellite marker technique combined with polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) , allelic frequency of inbred line swine can be calculated,from which polymorphic information content can be obtained so that gene homozygosity and isogenicity can be exactly confirmed at the molecular level, and all of which can provide reliable genetic bases for application of inbred line swine to such fields as transgenic research, xenotransplantation and studies as experimental animals.
【Fund】: 国家自然科学基金重点资助项目 (39830 1 90 )
【CateGory Index】: S828
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