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《Progress In Veterinary Medicine》 2004-06
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Manufacture and Quality Control of the Anti-porcine Reproductive and Respiratory syndrome virus IgG Injection

YA Zong-bao~(1), LI Liu-jin~(2), LI Ming-tao~(1), ZHU Qiu-xia~3,MA Guo-ji~1,TIAN Hong-xiong~4,LI Yong~(2),LIU Xue-tong~1 (1.Generale Station of Animal husbandry and Veterinary Medicine of Shaanxi,Shaanxi,Xi’an,710000,China;2.Laboratory Animal Center, Fourth Military Medical University,Xi’an,Shaanxi,710032,China;3.Yangling K.N.Y Veterinary Pharmaceutical Ltd.Company, Yangling,Shaanxi,712100,China;4.Station of Animal Husbandry and Veterinary Medicine of Yan’an,Yan’an,Shaanxi,716000,China)  
To prepare anti-porcine reproductive and respiratory syndrome virus IgG injection, we vaccinated GuanZhong donkey with inactivated porcine reproductive and respiratory syndrome virus antigen which is accompanied with adjuvant, according to basic immune and booster immune procedure.After 9~11 days,we extracted the blood plasma from the donkeys ,then isolating and,purificating as well as identification anti-PRRSV IgG.Finally,we carried out the serum neutralization test on the IgG ,the antibody value in the animal serum is reached over 1∶320,indicating that this method can successfully produce the anti-porcine reproductive and respiratory syndrome virus IgG injection.
【CateGory Index】: S852.5
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