Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress In Veterinary Medicine》 2005-01
Add to Favorite Get Latest Update

Sliver Staining of Microsatellite DNA-PAGE and Simpe Method of Making Dry Gel

HUO Jin-long,ZHANG Juan,LUO Gu-yue,PAN Wei-rong,ZHANG Mei,ZENG Yang-zhi(Banna Mini-pig Inbred Lines Key Laboratory of Yunnan Province,Kunming,Yunnan,650201,China)  
In order to fully separate DNA fragments after polymerase chain reaction(PCR)and enduringly preserve PAGE gel, 15 microsatellite primers on swine were used during expriment. First, making PCR; then, the fragments were separated by polyacrylamide gel electrophoresis (PAGE)and appeared by silver staining; last, the gels were processed for dry gels.The result indicated PAGE had very good effect on separating the discrepancy DNA fragments only few base pairs.The dry gel can be preserved for ever.This method especially apply for some laboratories without gel imaging system and gel drier.
【Fund】: 国家自然科学基金重点资助项目 ( 3 983 0 190 )
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 YE Kai-xin,LUO Sen-cai,LIANG Xue-lian,LIANG Hong(College of Life Sciences,Zhongkai University of Agricultural and Technology,Guangzhou 510225,China);SSR Analysis in Kiwifruit Cultivars[J];Biotechnology;2009-03
2 WANG Guang-li~1,SHI Pei-chun~1,ZHANG Wei~(1,2),CAO Lian-pu~(1,2),LI Ying-feng~1(1.College of Agronomy,Shihhotze University,Shihhotze 832003,China;2.Key Laboratory of Oasis Ecological Agriculture of Xinjiang Production and Construction Corps,Shihhotze University,Shihhotze 832003,China);Analysis and Explorations of Silver Staining Test of Non-denaturant Polyacrylamide Gel Eletrophoresis[J];Xinjiang Agricultural Sciences;2006-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHENG Bao-weng 1,CHENG Guo-di 2,ZHANG Hua-jian 1 (1.Yuxi Public Security Bureau of yunnan province, Yuxi, 653100,China; 2.Department of Forensic Biology,West China University of Medical Sciences,Chengdu,610041, China);A Study of Forensic Individual Identification Used STR Locus With Silver Staining and Multiplex PCR Methods[J];Hereditas(Beijing);2002-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHOU Chun-li et al(School of Life Science,Jiangxi Science & Technology Normal University,Nanchang,Jiangxi 330013);Comparison of Two Methods of Genomic DNA Extraction from Pork Liver[J];Journal of Anhui Agricultural Sciences;2009-18
2 HUANG Da-peng,CHEN Jian-hua (College of Animal Science and Technology,Heilongjiang August First Agriculture University,Daqing,Heilongjiang,163319,China);Comparing Five Kinds of Methods to Extact Genomic DNA from Whole Blood[J];Progress in Veterinary Medicine;2006-06
3 Fan Yuxin; Yu Long; Ren Mingshan;Sun Xiyuan; Hong Mingfan;Wu Mengchu;He Guangyuan;Han Yongzhu;Yang Renmin;Zhao Shouyuan(Fudan University, Anhui College of TCM);Preliminary study on mutations of the copper transporting P-type ATPase gene in the Chinese[J];JOURNAL OF FUDAN UNIVERSITY;1997-05
4 Ke Liuyu1,2 Xie Yanyan1 Xiao Tianfang1,2 Jiang Xiaobing2(1.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002;2.State gene pool of waterfowl breed resources,Shishi,Fujian 362700);Comparison of methods of DNA extraction from poultry blood[J];Fujian Journal of Animal Husbandry and Veterinary Medicine;2008-02
5 CHENG Bao-wen,ZENG Fa-ming,LU Fan,et al./Yunnan Security Department,Kunming 650021,China;Mitochondrial DNA polymorphism of mongolians populations in China and its forensic evaluation[J];Chinese Journal of Forensic Medicine;2007-02
6 CHEN Hong-ying1,2,CUI Bao-an1,2,LI Xin-sheng1,ZHANG Hong-ying1,WANG Dong-fang1,2,ZHANG Xiang1(1.College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China; 2.Henan Animal Food Safety Key Laboratory,Henan Province,Zhengzhou 450002,China);Diagnosis of porcine circovirus disease[J];Guangdong Agricultural Sciences;2007-09
7 FAN Jing, XIONG Yonghua, HU Na, YANG Hui, XU Yang Jiangxi-OAI Joint Research Institute, The Key Laboratory of Food Science of MOE, Nanchang, 330047, China;Study on Mutiplex Amplification of CSFIPO, TH01 and TPOX[J];Foreign Medical Sciences;2004-01
8 HONG Yan-yun1,2,LI Di-qiang2,YI Tu-yong1,ZHANG Yu-guang2,LIU Yi1(1.Hunan Agricultural University,Changsha 410128,China;2.Institute of Forestry Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China);Screening Microsatellite Primers and their Application for Individual Identification in Faecal DNA from Procapra praprzewalskii[J];China Animal Husbandry & Veterinary Medicine;2009-02
9 Zhang Kunhe Zhu Jiqun Huang Deqinang et al. The First Affiliated Hospital of Jiangxi Medical College Nanchang 330006 ;The analysis and resolving of the problem failing to amplify p53 gene by PCR in colonic cancer[J];JIANGXI JOURNAL OF MEDICAL LABORATORY SCIENCES;2000-03
10 SUN Qi,ZHANG Yingdong,LIU Yang.Department of Neurology,Affiliated Brain Hospital,Nanjing Medical University,Nanjing 210029,China;The association of PAI-1 gene(CA)n dinucleotide repeats in the 4th intron polymorphism with infarction[J];Journal of Clinical Internal Medicine;2006-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YANG Miao-xian et al(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225);Application of SSR Molecular Markers in Kiwifruit Genetic Diversity[J];Journal of Anhui Agricultural Sciences;2008-32
2 Yang Jun 1 Xu Kai 1 Yan Chunguo 1 Shen Caodong 2 (1.Department of Horticulture,Anhui Agricultural University,Hefei 230036; 2.Department of Horticulture,Zhejiang Agricultural University);Studies on Isozyme of Peroxidase in Some Plum Varieties[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-04
3 YAN Lu-Na,ZHANG De-Xing ** State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China;Effects of sample size on various genetic diversity measures in population genetic study with microsatellite DNA markers[J];Acta Zoologica Sinica;2004-02
4 Song Wan; Xu Jiuru. Journal of Beijing Forestry Universtity (2000)22(1)76~80 [Ch,24 ref.] College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China.;Progress in application of germplasm identification and fingerprinting of fruit trees.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-01
5 Zhang Junwei Bao Manzhu Chen Longqing (Department of Forestry, Huazhong Agricultural University, Wuhan,430070,P.R.China);Analysis of Prunus mume, P.persica, P.salicina, P.armeniaca, P.subhirtella and P.lannesiana by RAPD[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1998-02
6 CHENG Li-li~1,SU Shu-chai~1,QIN Ling~(2*),YIN Wei-lun~1(1.College of Resourses and Environment,Beijing Forestry University,Beijing 100083,China;2.Beijing Agricultural College,Beijing 102206,China);Extraction of DNA and Estabilishment of AFLP Techniques in Leaves of Castanea mollissima Blume[J];Journal of Beijing Agricultural College;2005-02
7 CHEN You chun, CAO Hong he, LI Hong bing (Chinese Academy of Agriculture Science, Beijing 100094, China);Application of Random-locating Sampling in Subpopulation of Animal Breed[J];Journal of Yellow Cattle Science;2001-01
8 LU Shen-jin 1, MA Yue-hui 2, GENG She-min 2, YANG Yan 1 (1.College of Animal Science and Technology, Northwest Sci-tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 2. Chinese Academy of Agriculture Science, Beijing, 10094 );Discussion on Genetic Distance Analysis Methods with Genetic Marker[J];Journal of Yellow Cattle Science;2002-05
9 ZHANG Li juan, ZHANG Bao jun, GENG She min(College of Animal Science and Technology, Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shannxi 712100,China);Microsatellite Marker and Amimal Breeding and Genetics[J];Journal of Yellow Cattle Science;2003-02
10 Jia Yonghong 1,2 Jian Chengsong 2 Shi Xianwei 1 ( 1 Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences Kunming 650223; \ 2 Animal Science Department in Guizh;Genetic Marker and Its Application in Animal Breeding[J];JOURNAL OF YELLOW CATTLE SCIENCE;1999-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Liang-ming,ZHANG Dong-lin,LI Zhi-hui,CHEN Yonghua,XIAO Jing-jing,PENG Li-mei (School of Resources and Environment,Central South University of Forestry and Technology,Changsha 410004,Hunan,China);Improvement on PAGE Silver Staining and Band Recovering[J];Journal of Central South University of Forestry & Technology;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 WANG Yong\|Qing ① WANG Xin\|Guo ② XU Lai\|Xiang ① ZHANG Zhi\|Bin ① (①Institute of Zoology,Chinese Academy of Sciences\ Beijing\ 100080; ②Institute of Biological Engineering\ Beijing\ 100071, China);A New Rapid Method for Extraction of High Quality of Genomic DNA from Animal Tissues[J];Chinese Journal of Zoology;2001-01
2 Jiang Xianhua Lu Shihui Wang Guolin Zhu Yuwen He Zhongnian Liaoning Criminal Scientific and Technical Research Institute;THE PRACTICAL USE OF THE DNA FINGERPRINT IN FORENSIC MEDICINE[J];Chinese Journal of Forensic Medicine;1990-01
3 LI Sheng-Bin, LAI Jiang-Hua, GAO Shu-Hui, ZHENG Hai-Bo, FENG Ji-Dong, ZHAO Jian-Min, LI Shuang-Ding, FENG Cheng-Bo, JIN Tian-Bo, WANG Jian, YANG Huan-Ming (1. Human Genome Center, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China;STR Polymorphisms in Five Chinese Ethnic Groups(2)[J];Acta Genetica Sinica;2000-12
4 ZOU Lang-ping (Institute of the Forensic Sciences Higher Court of Yunnan Province Kunming 650021) YANG Yan (Yunnan Provincial People's Hospital Kunming 650033) CHU Jia-you(Institute of Medical Biology Chinese Academy of Medical Sciences Kunming 650087) S;Distribution of CSF1PO,TPOX and TH01 Loci in Han Chinese[J];ACTA GENETICA SINICA;1998-03
5 HUANG Dai-xin,ZHANG Lin,WU Mei-yun,CHEN Guo-di,CHEN Yu-bo (Department of Forensic Biology, West China University of Medical Sciences, Chengdu 610041, China);Population Genetic Study of the STR Locus D7S2201 in Chinese and Thai Populations[J];Hereditas(Beijing);2001-02
6 HOU Yiping 1, LI Yingbi 1, TANG Jianping 1, WU Jin 1, ZHANG Sizhong 2, ZHANG Ji 1, ZHU Jiayou 3, Mechthild Prinz 4. 1(Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P. R. China. E mail: rech;Genetic polymorphisms of eight STR loci in a Chinese Han population[J];CHINESE JOURNAL OF MEDICAL GENETICS;2000-04
7 HOU Yiping*,WU Jin, LI Yingbi,TANG Jianping, ZHANG Sizhong, CHU Jiayou,M. Prinz.*Institute of Forensic Medicine, West China University of Medical Sciences, Chengdu, 610041 P.R.China. Email:rechtsme@wcums.edu.cn;Genetic polymorphisms of five STR loci in a Chinese Han population[J];CHINESE JOURNAL OF MEDICAL GENETICS;1999-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved