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《Progress In Veterinary Medicine》 2005-01
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Sliver Staining of Microsatellite DNA-PAGE and Simpe Method of Making Dry Gel

HUO Jin-long,ZHANG Juan,LUO Gu-yue,PAN Wei-rong,ZHANG Mei,ZENG Yang-zhi(Banna Mini-pig Inbred Lines Key Laboratory of Yunnan Province,Kunming,Yunnan,650201,China)  
In order to fully separate DNA fragments after polymerase chain reaction(PCR)and enduringly preserve PAGE gel, 15 microsatellite primers on swine were used during expriment. First, making PCR; then, the fragments were separated by polyacrylamide gel electrophoresis (PAGE)and appeared by silver staining; last, the gels were processed for dry gels.The result indicated PAGE had very good effect on separating the discrepancy DNA fragments only few base pairs.The dry gel can be preserved for ever.This method especially apply for some laboratories without gel imaging system and gel drier.
【Fund】: 国家自然科学基金重点资助项目 ( 3 983 0 190 )
【CateGory Index】: Q78
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