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《Progress In Veterinary Medicine》 2005-03
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Application of Flow Cytometry in the Study of Apoptosis

RUAN Hong-mei,ZHANG Qi-ya (Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan,Hubei,430072,China)  
As a new developing biotechnology, flow cytometry has been widely applied in the animal medical field include cell biology, virology, oncology, immunology, pathology and so on. Flow cytometry has the advantages of rapid, exactly and multiparameter analysis of large populations of individual cells to investigate the biological processes associated with apoptosis. Based on the morphological, biochemical and molecular changes during apoptosis, including cellular shrinkage, permeability transition of plasma membrane, caspases activation, dissipation of mitochondrial transmembrane potential, phosphatidylserine redistribution, calcium flux, DNA fragmentation.The review outlined the methods to characterize, identify and quantify apoptotic cells by flow cytometry.
【Fund】: 863计划项目(2001AA626030.2002AA626010);; 国家自然科学基金项目(30170726);; 中国科学院创新资助项目(KSCX2 SW 302)
【CateGory Index】: Q255
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