Effect of LPS on Na~+-K~+-ATPase on the RBC Membrane in Goats with Endoxemia
XU Jia-hua,WU Xuan-guang,ZOU Wan-sheng,WU Feng-ming(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China)
The purpose of this experiment was to determine the effect of endotoxin(LPS) on Na~(+)-K~(+)-ATPase and explore the action mechanisns of LPS in the goats with endoxemia.The healthy goats(10 kg±(1 kg)) were randomly divided into two group: saline group(n=6) and LPS group(n=6).The saline group and LPS group received saline and Lipopolysaccharide(1 mg/kg) through neck vein,respectively.The blood samples were collected at 0,0.5,1,2,3, 4,5 h and 6 h after treatment,then separated red blood cell and serum from the sample and made red blood cell membrane.Na~(+)-K~(+)-ATPase in the red blood cell membrane and the K~(+) concentration in the red blood cell and serum were measured.It showed that Na~(+)-K~(+)-ATPase(LPS group) initially became more active,then repressed.Concentration of K~(+) in the red blood cell initially increased,then decreased.On contrast,concentration of K~(+)in the serum initially decreased and then increased.
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