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《Progress in Veterinary Medicine》 2006-01
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Synthesis of an Antigen and Preparation of Polyclonal Antibody for Sulfamethoxazole(SMZ)

SHEN Hong, WU Guo-juan, LI Huan-rong (Department of Animal Medicine and Sciences, Beijing Agricultural College, Beijing,102206,China)  
A polyclonal antibody (pAb) specific for sulfamethoxazole (SMZ) was prepared. Hapten SMZ was coupled with carrier protein human serum albumin (HSA) to form an antigen SMZ-HSA by diazotization method. The conjugates used to immunize New Zealand white rabbit were identified with UV scanning spectrum and high performance liquid chromatography (HPLC) and the couple rate of the antigen was 19∶1. After the antisera were obtained and purified using a modified n-caprylic acid-saturated ammonium sulfate method,the specificity of pAb was detected by indirect enzyme-linked immunosorbent assay (ELISA). It showed that the titer of pAb was 1∶10~(5 )and the detection limit in term of 50% inhibition rate was 22.53 ng/mL in the linear range of 0.1 ng/mL~10 μg/mL. The cross-reaction rate between SMZ and the pAb was 100% and the cross-reaction was very lower between the pAb and seven kinds of sulfonamides. The results showed that the anti-SMZ pAb had a high specificity and was good enough for immunoassay of SMZ in edible animal products.
【Fund】: 北京市农委资助(20020103);; 北京市教委资助(200310020084);; 北京市组织部资助(20041D0502106)
【CateGory Index】: S852.43-3
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