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《Progress in Veterinary Medicine》 2006-05
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The Determination of 4 Parameters Influencing the NK Cytotoxic Index

CONG Li-yuan~1,2,SONG Chang-xu~1,CHEN De-kun~2,ZHANG Chun-hong~1(1.Veterinary Medicine Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong,510640,China;2.College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi,712100,China)  
In order to determine discuss whether 4 factors,the ratio of effector cells and target cells,time of incubation and color precipitation,and the FCS concentration supplemented in maintenance medium,can infect NK cytotoxic index of mice spleen,we used LDH release method in this study.The results showed that the best ratio of effector cells and target cells was 50∶1,the reasonable time of incubation was 2 h.Under these conditions,maintenance medium with 50 mL/L FCS and 0.5 h for precipitation time were better for the NK cell activity.Therefore,the 4 factors can infect the NK Cytotoxic in different degree.
【CateGory Index】: S852.4
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