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《Progress in Veterinary Medicine》 2007-10
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Establishment of Mouse Bioassay for Detecting Ciguatoxin

ZHU Hai1,ZHENG Lu1,2,WANG Ai-hui1,3,FAN Fang1,GUO Ai-zhen2,LI Jin-feng1,2,YANG Xiao-xia1,ZHAO Fang1 (1.Food Inspection and Quarantine Center,Shenzhen Entry-exit Inspection and Quarantine Bureau,Shenzhen,Guangdong,518067,China;2.Key State Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan,Hubei,430070,China;3.Biology of Red Tide Lab,Life Science College,South China Normal University,Guangzhou,Guangdong,510631,China)  
This study was aimed to establish a mouse bioassay for ciguatoxin detection.Different concentrations of ciguatoxin P-CTX-1 were injected into Kunming strain mice by intraperitoneal route,and the toxic symptom was observed to determine the dosage for one mouse unit(mu) and develop the curve of dosage versus death time.The results demonstrated that the dosage of ciguatoxin for one mu is about 9.10 ng from 8.67 ng to 9.56 ng within 95% of confidence interval.The curve of dosage versus death time in male Kunming strain mice was represented by the equation y=244.9 x-1.393 7,where x means the toxin dosage(mu),while y means lethal time(t).The curve could be transferred into a straight line when the ciguatoxin dosage(mu) was expressed by logarithm(logmu) and the death time(t) by reciprocal of death time(1/t).The straight line was represented by the equation y=1.207 6 x-0.047,R2=0.992 1,where x means reciprocal of death time(1/t),while y means logarithm of ciguatoxin toxicity(logmu) and R2 means the regression coefficient.The method was validated with the toxin standard at concentrations of 0.1,0.2,0.3 ng/g.As a result,the average toxin recovery rate with this method varied from 46.58% to 50.23%.
【Fund】: 国家质检总局科技项目资助(2006IK126)
【CateGory Index】: X174
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