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《Progress in Veterinary Medicine》 2008-11
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Establishment of RT-PCR for Detecting the Inactivated Antigens of Peste des petits ruminants virus and Its Related Sequence Analysis

ZHAO Wen-hua,YANG Shi-biao,ZHU Jian-bo,XIAO Lei,LI Hua-chun,ZHANG Nian-zu(Key Opening Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture,Yunnan Academy of Animal Husbandry and Veterinary Medicine,Kunming,Yunnan,650224,China)  
In this study,the inactivated PPRV antigen was used as study material.Its genome RNA was extracted as the RT-PCR template.According to references and the downloaded sequences,three sets of primers of F gene were designed.Then RT-PCR amplification was performed and the amplified fragments were cloned to the pMD18-T vector,sequenced and analyzed.The results showed that the desired fragments can be amplified by the designed primers whether in the RT-PCR or the nested RT-PCR methods.Through sequencing analysis,the amplified fragments were correct and all sited in the F gene fragments.The primers used in this study are special to PPRV and can't amplify the viruses of the same genus,RPV and canine distemper virus(CDV).The methods can be used to differentiate the alike viruses.
【CateGory Index】: S854.43
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