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《Progress in Veterinary Medicine》 2009-05
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Establishment and Application of Double PCR Detection Method for Salmonella and Shigella

PAN Bao-jin1,LUO Zhao-fei1,WEI Mei-liang1,WANG Wen-long1,LIU Jun-yi1,CHEN Li-biao2 (1.Technology Centre of Guangxi Entry-Exit Inspection and Quarantine Bureau,Nanning,Guangxi,530021,China;2.Dongxing Entry-Exit Inspection and Quarantine Bureau,Dongxing,Guangxi,535651,China)  
Two pair of specific primers for the polymerase chain reaction(PCR) according to the relevant nucleotide sequences of Salmonella invA and Shigella ipaH in GenBank were designed.Double PCR method was established to detect DNA of Salmonella and Shigella in one reaction system.A 284bp DNA fragment of Salmonella and a 611 bp of Shigella were found,while the nagtive strains were not found.The sequences of amplified fragments were detected and compared with all relevant genes.The nucleotide homologies of Salmonella and Shigella were 93%-100% and 98%-100%,respectively.Double PCR method was established to detect Salmonella and Shigella of laboratory simian feces samples from 6 farms in Guangxi.In total of 1665 samples,25 were Salmonella positive,with a 1.5 percent positive rate;90 were Shigella positive,with a 5.4 percent positive rate.The double PCR technique had high specificity and sensitivity,and was suitable for detecting clinical samples.
【Fund】: 国家质检总局科技兴检项目(2007IK034)
【CateGory Index】: R450
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