Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress in Veterinary Medicine》 2009-06
Add to Favorite Get Latest Update

Advance in PCR-SSCP and Its Application

ZHOU Ming-tao,GAO Hong,XIAO Peng,ZHOU Hui(College of Animal Science Technology,Yunnan Agricultural University,Kunming,Yunnan,650201,China)  
PCR-SSCP is a method which detects gene mutations.Since its inception,it has undergone its own development and improvement.Initially,it puts isotope into PCR amplification of objects,and then shows results by autoradiography.But it also takes the difficulties to the technology to extend.With the DNA silver staining method and PCR-SSCP combined,especially the application of the direct ethidium bromide-staining method,it makes PCR-SSCP greatly simplified,primitively operate,lessly limitate,low-requirement experimental conditions for general clinical laboratory.It is simple,rapid,sensitive in character.PCR-SSCP is not only used to the detection of gene mutation and short sequence deletion and insertion,but also used to animal breeding,microbes,parasite research fields.Because of the advantages of SSCP,it has been applied widely in recent years.
【CateGory Index】: R450
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 YI Hong-mei~(1,2),WANG Feng-ge~1,ZHAO Jiu-ran~1,WANG Lu~1,GUO Jing-lun~1,YUAN Ya-ping~3(1.Maize Reaserch Center,Beijing Academy Agricultural and Forestry Science,Beijing 100089,China;2.Capital Normal University,Beijing 100089,China;3. Department of Plant Science,Jilin University,Changchun 130062,China);Comparison of Two Maize SSR Detection Methods: Capillary Electrophoresis with Fluorescence Detection Method and Denaturing PAGE Silver-staining Detection Method[J];Acta Agriculturae Boreali-Sinica;2006-05
2 MI Wen-xiu,XIE Bing,XU Ya-tong(Tiantong National Station of Forest Ecosystem,Key Laboratory of Urbanization and Ecological Restoration of Shanghai,Department of Environment Science,East China Normal University,Shanghai 200062,China);Analysis of Microbial Community in a Deodorization Biofilter by PCR-SSCP Method[J];Environmental Science;2008-07
3 WANG De-cheng~1,GAO Hong~1,FAN Quan-shui~2,GAO Bin~3(1.College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;2.The Military Medical Institute of Chengdu Military Area,Kunming 650032,China;3.College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China);Analysis of Polymerase Chain Reaction-Single Stranded Conformation Polymorphism on Olaquindox-Induced mtDNA Gene Damage in Chicken[J];Journal of Jilin Agricultural University;2007-01
4 BO Qi-fu1 , WANG Xiao-li2 , LI Ruo-bao1, WANG Jin-ping1 , LI Hong-xian1 (1.Department of Human Anatomy,Weifang Medical College 261042;2. Department of pharmacopedics of People's Hospital, Weifang 261041);The Mutation of p53 and K-ras Genes in Smoking Rats' Lung[J];Progress of Anatomical Sciences;2007-04
5 JIA Hai, JIA Zheng-ping, Fu Min-qiang, WANG Rong, CHEN Qiao-yun, XIE Hua, MA Jun ( School of Pharmacy, Lanzhou University, Lanzhou, 730000, China; Department of Pharmacy, Lanzhou General Hospital of PLA, Lanzhou, 730050, China; Lanzhou Institute of Biological Products, Lanzhou, 730046, China);Detection of vector gene mutation by single strand conformation polymorphism with capillary electrophoresis[J];Journal of Lanzhou University(Medical Sciences);2006-02
6 WANG Li-yun1,WANG Jin-yu1,CHEN Kuan-wei2,YU Jia-hui1,YANG Yan1,ZHANG Gen-xi1(1.Animal Science and Technology Department,Yangzhou University,Yangzhou 225009,China;2.Institute of Poultry,Chinese Academy of Agriculture Sciences,Yangzhou 225003,China);PCR-SSCP Analysis on Ghrelin Gene in Yellow Chicken of Two Breeds[J];Journal of Agricultural Biotechnology;2007-04
7 WANG Na, LIAN Yi-shui, LIU Yan-xing (Pharmacology Department, Basic Medical College, Hebei Medical University, 050017, China);Optimization of PCR-SSCP analysis method[J];Progress in Modern Biomedicine;2008-10
8 Li Yumei1 Yao Jiyuan2 Wu Jing2 Bai Xiujuan1(1Northeast Agriculture University Heilongjiang,Harbin 150030;2Jilin Agriculture University,Changchun 130118);Research and Application Development of PCR-SSCP Technology[J];Biotechnology Bulletin;2007-06
9 XUE Yan,CHANG Hong,CHANG Guo-bin((College of animal science and technology,Yangzhou University 225009);PCR-SSCP Technique and Its Advances in Research of Animal Breeding[J];Journal of Animal Science and Veterinary Medicine;2005-03
10 WANG Yan-yan,ZHANG Hui,XIANG Wen-sheng(1.College of Life Science,Northeast Agricultural University,Harbin 150030,China;2.Insitute of Crop Germplasm Resources,Chinese Academy of Agricultural Sciences,Beijing 100081,China);PCR-SSCP Technique and Its Application in Botanical Study[J];China Journal of Bioinformatics;2008-01
Chinese Journal Full-text Database 10 Hits
1 LIU Yuan-yuan et al (Life Science College,Lanzhou University,Lanzhou,Gansu 730000);Rapid Detection on the Enzyme-digested Products of Restriction Endonuclease by Using Capillary Electrophoresis[J];Journal of Anhui Agricultural Sciences;2008-10
2 XIE Xiao-zhen,LI Yang,QIU Zong-wen(Department of Clinical Labortary,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);A study on clincal distribution and bacterial resistance of coagulase negative staphylococci[J];Chongqing Medicine;2006-17
3 Zhao Zhao-xia1,Liu Wen-zhong1,Zhu Wen-jin2,et al(1. College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,China;2. Department of Animal Science,Hebei Normal College of Science and Technology);Analysis on the Genetic Diversity of Partial Sequences in Mitochondrial DNA D-loop of Six Donkey Breeds[J];China Herbivores;2008-04
4 Wang Fengge~* Yi Hongmei~* Zhao Jiuran~(**) Wang Lu Maize Research Center,Beijing Academy of Agricultural and Forestry Sciences,Beijing,100097;Analysis of the Abnormal Peak Types in Capillary Electrophoresis with Fluorescent-labeled[J];Molecular Plant Breeding;2007-06
5 WANG Yong WANG Jun-yun National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,China;Application of molecular methods in identification and phylogeny of Leishmania[J];International Journal of Medical Parasitic Diseases;2006-05
6 LI Jun-ying1,ZHAN Kai1,XU Yue-ying1,WANG De-sheng2,ZHAO Rui-hong1,TANG Zhong-jie3(1.Institute of Animal Husbandry and Veterinary,Agricultural Academy of Anhui Province,Hefei 230031,China;2.Haian Town Livestock Farm Veterinary Station of Haian County of Jiangsu Province,Haian 226600,China;3.Chaohu Duck Breeding Farm of Lujiang County of Anhui Province,Lujiang 231490,China);PCR-SSCP Detection of Ghrelin and Pit-1 Gene in Chaohu Duck[J];China Animal Husbandry & Veterinary Medicine;2009-05
7 ZOU Jia-jie,ZHANG Ting,TANG Shu-sheng,CHEN Qian,JIN Xi,CHEN Kai-pao,XIAO Xi-long(Department of Pharmacology and Toxicology,College of Veterinary Medicine,China Agriculture University,Beijing 100193,China);Oxidative Damage of Mitochondria by Olaquindox in HepG2 Cells[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-05
8 YIN Rong-huan1,BAI Wen-lin 1,FANG He-chun 2,WANG Jian-min1,PAN Shu-de1,LIU Bao-shan1,LIU Jin-ling1,JIANG Zhuo1(1.College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang 110161,China;2.Disease Center for Prevention and Control in Liaozhong County of Liaoning Province,Liaozhong 110200,China);Effect of Olaquindox on Inducing Apoptosis of Testicle Spermatogenic Cell in Mouse[J];Acta Agriculturae Boreali-Sinica;2008-01
9 Liu,Yuanyuana,b,c Wang,Rong,a Jia,Zhengpinga Guo,Zhiqianga Xin,Xiaotinga,c Xie,Huaa Ma,Juna Wang,Juana Li,Wenbina(a Department of Pharmacy,Lanzhou General Hospital of PLA,Lanzhou 730050)(b Gansu Red Cross Blood Center,Lanzhou 730046)(c Life Science College,Lanzhou University,Lanzhou 730000);H-ras Gene Mutation Detection of Gastric Cancer by Restriction Fragment Length Polymorphism with Capillary Electrophoresis[J];Acta Chimica Sinica;2009-04
10 ZENG Yan-linga,TAN Xiao-fenga,ZENG Xiao-fenga,b (a.The Key Lab of Non-wood Forest Products of Forestry Ministry;b.Office of Enrollment and Employment,Central South University of Forestry and Technology,Changsha 410004,Hunan,China);Single Nucleotide Polymorphism Genotyping and Its Application in Woods Breeding[J];Nonwood Forest Research;2009-01
China Proceedings of conference Full-text Database 1 Hits
1 LONG Xing~(1,2),XIA Rui~1,HUANG Yong-hong~1,ZENG Ji-wu~(1*),YI Gan-jun~(1*),HUANG Bing-zhi~1,and CHEN Jin-yin~2 (1 Fruit Research Institute,Gongdong Academy of Agricultural Science,Guangdong 510640 China;2 College of Agronomy,Jiangxi Agricultiral University,Nanchang 330045 China);Application of Capillary Electrophoresis in The Study on Relationships of Banana Varieties by AFLP[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Dong Chengliang Deng Changyan (The College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Progress of Examination Techniques for Gene Mutation[J];JOURNAL OF YELLOW CATTLE SCIENCE;1998-03
2 Wang Wen Shi Liming (Laboratory of Celluar and Molecular Evolution, Kunming Institute of Zoology, Academia Sinica 650223);AN IMPROVED METHOD FOR ISOLATION OF ANIMAL MITOCHONDRIAL DNA[J];Zoological Research;1993-02
3 WEI Tai yun, LIN Han xin, XIE Lian hui (Institute of Plant Virology, Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002, China);Optimization of the conditions affecting PCR-SSCP analysis[J];Journal of Fujian Agricultural University(Natural Science);2002-01
4 Shi Wei, Zhang Yukui, Chen Nong, Zhang Jin, Wang Lei, Zou Hanfa (Dalian Institule of Chemical Physics, Chinese Academy of Sciences, National Chromatographic R&A Center , Dalian 116011);Preparation and Characterization of High Performance Linear Polyacrylamide Capillary Gel Electrophoresis Columns and Investigation of the Experimental Conditions[J];Chinese Journal of Analytieal Chemistry;1998-01
5 Shi Xianzhe 1,Li Jianhua 2,Zhao Chunxia 1,Lü Shen 2,Yang Qing 1,Xu Guowang *1 1 (National Chromatographic Research & Analysis Center,Dalian Institute of Chemical Physics, Chinese Academy of Sciences,,Dalian 116011) 2 (The Secondary Affiliated Hospital of Dalian Medical University, Department of Pathology, Dalian 116027);K-ras Gene Mutation Detection by Single-Strand Conformation Polymorphism with Capillary Electrophoresis[J];Chinese Journal of Analytical Chemistry;2005-02
6 ZHAO Yang-guo, REN Nan-qi, WANG Ai-jie, LIU Guang-min, ZHAO Qiu-shi, SHANG Huai-xiang (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Microbial Community Structure Analyzed by Single-Strand Conformation Polymorphism Technique in Sulfate-Reducing Reactor[J];Environmental Science;2005-04
7 Wu Zhenfang 1) Xiong Yuanzhu 1) Deng Changyan 1) Jiang Siwen 1) I.Harbitz 2) ( 1) Huazhong Agricultural University, Wuhan 430070; 2) Dept of Biochemistry, Physiology and Nutrition, Norwegian College of Veterinary Me;STUDY ON THE POLYMORPHISM OF PORCINE LIPOPROTEIN LIPASE GENE AND SEQUENCING THE PARTIAL DNA FRAGMENTS[J];JOURNAL OF HUAZHONG AGRICULTURAL;1999-05
8 Zheng Xueli\ \ Hu Xiaosu\ \ Chen Jianping\ \ Zhang Tao(Laboratory of Parasitology, West China University of Medical Sciences,Chengdu 610044);ANALYSIS OF SUU rRNA GENE AND kDNA OF CUTANEOUS LEISHMANIASIS PATHOGENFROM XINJIANG OF CHINA BY PCR SSCP*[J];ACTA PARASITOLOGICA ET MEDICA ENTOMOLOGICA SINICA;1998-03
9 GUO Xiao-quan, HU Guo-liang, ZHANG Cai-ying(College of Animal Science and Techonology, JAU, Nanchang 330045, China);Exprimental Study on Clinical Pathology of Poisioning of Olaquindox in Chongren Chickens[J];Acta Agriculturae Universitis Jiangxiensis;2003-02
10 WT5BX]LU Meng zhu 1, WANG Xiao ru 2, Alfred E.Szmidt 2 (1.Research Institute of Forestry,CAF,Beijing 100091,China; 2.Department of Forest Genetics,SLU,Umea 90183,Sweden);Application of PCR SSCP in Genetic Analysis of Conifers[J];FOREST RESEARCH;2000-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved