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《Progress in Veterinary Medicine》 2009-06
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Advance in PCR-SSCP and Its Application

ZHOU Ming-tao,GAO Hong,XIAO Peng,ZHOU Hui(College of Animal Science Technology,Yunnan Agricultural University,Kunming,Yunnan,650201,China)  
PCR-SSCP is a method which detects gene mutations.Since its inception,it has undergone its own development and improvement.Initially,it puts isotope into PCR amplification of objects,and then shows results by autoradiography.But it also takes the difficulties to the technology to extend.With the DNA silver staining method and PCR-SSCP combined,especially the application of the direct ethidium bromide-staining method,it makes PCR-SSCP greatly simplified,primitively operate,lessly limitate,low-requirement experimental conditions for general clinical laboratory.It is simple,rapid,sensitive in character.PCR-SSCP is not only used to the detection of gene mutation and short sequence deletion and insertion,but also used to animal breeding,microbes,parasite research fields.Because of the advantages of SSCP,it has been applied widely in recent years.
【CateGory Index】: R450
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