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《Progress in Veterinary Medicine》 2010-05
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Cloning and Sequence Analyses of the NSP2 and ORF7 Genes of PRRSV Strains in Anhui Province

YAO Xue-jing1,ZHAN Song-he2,ZHU Liang-qiang2,QI Ke-zong1,WANG Fei2,WANG Ai-ping1 (1.College of Animal Science and Technology,Anhui Agricultural Univresity,Hefei,Anhui,230036,China;2.Anhui Centre for Animal Diseases Control and Prevention,Hefei,Anhui,230022,China)  
Samples were collected from two farms in Anhui province with suspected clinical signs of porcine reproductive and respiratory syndrome in 2008,and two porcine reproductive and respiratory syndrome virus isolates(HS08 and ZJ08) were isolated after 4-5 passages in Marc-145 cells.The NSP2 and ORF7 genes of the isolates were amplified using RT-PCR which was followed with cloning and sequencing.The two isolates were similar to each other(nucleotide homology 99.0%),and they contained a discontinous 30 amino acid deletion in the NSP2 gene.The two isolates had 80.3% and 80.2% nucleotide homology with strains CH-1a,respectively,in the NSP2 gene.They had 98.4% and 99.5% nucleotide homology with the highly pathogenic strain HuN4 isolated in 2007 in the ORF7 gene,and they had mutations of K46→R46,H109→Q109,V117→A117 in the ORF7 gene.Phylogenetic analysis also revealed that the two isolates belonged to the cluster of highly pathogenic strains emerging in China in recent years.
【Fund】: 安徽省科技攻关项目(0810302150)
【CateGory Index】: S852.659.6
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