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《Progress in Veterinary Medicine》 2010-07
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Study on the Growth Characteristic of Immortalized Swine Vascular Endothelial Cells Infected with Lapinized Chinese Strain of Classical Swine Fever Virus

TAN Xiao-ni,ZHANG Yan-ming,ZHANG Xu,DENG Li (College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China)  
The 70th immortalized swine vascular endothelial cells was inoculated with lapinized Chinese strain(c-strain) of classical swine fever virus(CSFV).The cultural supernatants were collected for virus detection and the infected cells were passaged culture in the 72 h after inoculation.The morphological character of infected cells and mock cells were observed and the HCLV was determined in the 70th,75th,80th,90th,100th and 105th generations infected cells.The results showed that the morphological character of infected cells were similar with normal cells,but the swine vascular endothelial cells that were spreaded to 35th(the 105th SUVEC) after inoculation,became slightly elongated.HCLV were determined in each generation experimental infected cells.This study has laid a foundation for HCLV propagation in SUVEC.
【Fund】: 国家863计划项目(2006AA10A204)
【CateGory Index】: S858.28
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