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《Progress in Veterinary Medicine》 2010-07
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Establishment of A PCR for Identifying the Virulent Strain and Attenuated Vaccine Strain of MDV

WANG De-sheng1,2,LUO Ming-xing1,SHI Kai-zhi1,ZENG You-ling1,LI Yong-ming1,2 (1.College of Animal Science,Guizhou University,Guiyang,Guizhou,550025,China;2.Institute of Animal Disease,Guizhou University,Guiyang,Guizhou,550025,China)  
A pair of primers were synthesized to flank the 132 bpr tandem direct repeats in the serotype 1 of Marek's disease virus(MDV) genome.The polymerase chain reaction(PCR) was applied to detect the liver materials of suspicious MDV affected chickens in clinical cases and the feather pulp samples of healthy chicks inoculated with CVI988 vaccine at 1 day age.The results showed that out of 10 liver materials,7 amplified a 314bp DNA fragment,corresponding to two copies of the 132bpr tandem direct repeats.The PCR spectrum was in accordance with CVI988 attenuated vaccine,when the feather pulp samples were PCR-amplified,corresponding to 6-8 or more copies of the 132bpr tandem direct repeats.According to the discrepancy of PCR spectrum,the virulent MDV strain could be differentiated from CVI988 attenuated vaccine strain.The feather pulps were collected as the samples and the efficacy of chicks inoculated with serotype 1 vaccine was also surveyed in this experiment.
【Fund】: 贵阳市农业局科学技术计划项目([2008]动防-01号)
【CateGory Index】: S854.43;S852.5
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