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《Progress in Veterinary Medicine》 2011-04
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Study on Elimination of Drug-resistance Plasmid of E.coli

LI Ji-zong1,ZHOU Bi-jun 1,2,MA Zhe1,PENG Quan-hai1,GAO Ying1,ZHANG Ren-jun1,WANG Kai-gong1,2,WEN Ming1,2*(1.College of Animal Science of Guizhou University,Guiyang,Guizhou,550025,China;2.Animal Disease Institute of Guizhou Province,Guiyang,Guizhou,550025,China)  
To understand the drug resistance and the other physiological property after plasmid elimination treatment of E.coli.The E.coli with plasmid containing aac(6')-Ib gene was used for drug sensitivity test.The drug sensitivity showed that the strains almost resisted all of the 8 antibiotics including Aminoglycosides and Quinolones.In this study one strain of highest drug-fast was reversed by high temperature and SDS.PCR results showed that the resistant gene named aac(6')-Ib was eliminated,and the drug resistance was disappeared.When the strain was cultured without antibiotic,the drug sensitivity was constant.But the environment of kanamycin could recover the drug resistance after 4 generations.The strain without drug-resistance plasmid growed faster,and went into logarithmic phase earlier.Therefore,drug resistance of E.coli caused by plasmid.The strategy to eliminate the plasmids would be an effective way to deal with the multiresistance issue.
【Fund】: 贵州省科技厅农业科技攻关项目(黔科合NY字[2009]3069号)
【CateGory Index】: S858.28
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