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《Progress in Veterinary Medicine》 2014-11
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Establishment of Concentration Determination of Staphylococcus aureus by Spectrophotometry

XIAO Min;YANG Feng;WANG Xu-rong;LUO Jin-yin;LI Xin-pu;CHEN Jiong-ran;LI Hong-sheng;Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS,Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture,Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province;  
This study aimed to establish a method to measure the Staphylococcus aureus antigen concentration in multiple vaccine involved in bovine mastitis.Staphylococcus aureus colony in blood agar plate was washed down and diluted to different bacterial suspension.The bacterial counts and absorbance(A450)were determined by plate count method and spectrophotometer method,respectively.The correlation between bacterial counts and absorbance(A450)was studied.The result suggested that Staphylococcus aureus counts and absorbency showed linear correlation.a regression equation was established between Staphylococcus aureus bacterial suspension concentration and absorbancy(y=8.410 6x-0.272 6,R2=0.975 8).The bacterial concentration can be calculated quickly by measuring the absorbancy of Staphylococcus aureus.Four batch of Staphylococcus aureus were used to compare and verify the plate count method and spectrophotometer method,which showed that the spectrophotometer method was more simple,quick,and exact,and batter than that of plate count method,suggesting that it can be used to detect Staphylococcus aureus antigen concentration in multiple vaccine involved in bovine mastitis.
【Fund】: “十二五”国家科技支撑计划(2012BAD12B03);; 甘肃省农业科技创新项目(GNCX-2013-59);; 甘肃省农业生物技术研究与应用开发项目(GNSW-2013-28)
【CateGory Index】: S852.611
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