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《Progress in Veterinary Medicine》 2017-07
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Development of RT-PCR for Detection of Peste-des-petits Ruminants Virus in Chasmere Goats of Norshwest Shaanxi

HAO Yu-qing;LIU Jian-peng;YANG Zeng-qi;Animal Disease Prevention and Control Center of Yulin;College of Veterinary Medicine,Northwest A&F University;  
In order to establish a rapid molecular biological method for testing peste des petits ruminants virus,the N gene sequence of peste des petits ruminants virus in NCBI database was analyzed,the specific primers were designed based on this gene,a one-step RT-PCR method for the detection of N gene of peste des petits ruminants ruminants virus in this area was established.The pair of primers were used to detect different samples from epidemic focus,the detected positive bands were sequenced,it can be determined that the epidemic strains and state released strains have the same genotype.The establishment of this detection method is propitious to the active monitoring and passive monitoring of the peste des petits ruminants epidemic situation,the technical support will be provided to prevent and control the peste des petits ruminants epidemic situation.
【Fund】: 农业科技创新转化资金项目(NYKJ-2015-023);; 陕西省科技统筹创新工程科技技术项目(2015KTTSNY04-04)
【CateGory Index】: S852.654
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Chinese Journal Full-text Database 7 Hits
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【Co-citations】
Chinese Journal Full-text Database 7 Hits
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