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《Journal of Chinese Electron Microscopy Society》 2006-05
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TEM observation of Polysaccharide from Root of Paeonia suffruticosa Andr

DAI Ling~1,SHEN Ye-shou~1,ZHUANG Yong-long~2,ZHAO Zhi-ping~1,LIAO Hong-mei~1(1 School of Life Science,Anhui University,Anhui Key Laboratory of Eco-engineering & Bio-technique,Hefei Anhui 230039;2 Modern Experiment Center,Anhui University,Hefei Anhui 230039,China)  
The ultrastructure of polysaccharide isolated from the root of Paeonia suffruticosa Andr named as PSM2b-A was examined by transmission electron microscopy.PSM2b-A samples in aqueous solution were heated at 45℃ for 20 minutes before dipping to the copper grids following negative staining by uranyl acetate after air-dried.Coiled balls or circles,extended chains,network-like structures have been observed.It is presumed that polysaccharide molecules of PSM2bA in aqueous solution are likely to coil into floppy or compact globular structures which may assemble into aggregates by means of hydrogen bonds,leading to a variety of morphology.The TEM images provide useful insight into the conformations and interactions of polysaccharide molecules.
【Fund】: 安徽省自然科学基金资助项目(03043301);; 安徽大学211工程学术创新团队资助项目
【CateGory Index】: R284
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