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《Journal of Chinese Electron Microscopy Society》 2013-03
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The improvement of bacteria sample preparation method for scanning electron microscope observation

LIANG Jiang-nan,LIU Yi-wei,XIE Jia-yi(Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)  
The conventional SEM sample preparation needs multiple steps including fixing,rinsing,and dehydrating.Because the volume of microbiology sample or unicellular sample is so small,so each step of preparation needs centrifugal collection.However,multiple centrifuging may cause loss of the sample.In this study,filter paper pack is used to collect sample.This method significantly improves the efficacy of the sample preparation and enhances the observing effect of the sample.
【CateGory Index】: Q254
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIANG Jing-nan,LIU Yi-wei*(Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China);The improvement for the phenomena of phosphate crystallization during making microbiological samples for scanning electron microscope observation[J];Journal of Chinese Electron Microscopy Society;2012-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZOU Ru-yi et al (Chemistry Department,Shangrao Normal College,Shangrao,Jiangxi 334001);Effects of Non-Gilded Escherichia coli under Scanning Electric Microscopy[J];Journal of Anhui Agricultural Sciences;2009-32
2 JI Qing-zhan et al(College of Biological Science and Engineering,North Nationalities University,Yinchuan,Ningxia 750021);Research on the Observation Method of the Bacterial Isolate of DFRKN-1 with Scanning Electron Microscope[J];Journal of Anhui Agricultural Sciences;2011-10
3 LIU Jian-nan,WANG Ping,YIN Ming-rui,WANG Lei(College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China);ISOLATION AND IDENTIFICATION OF A HETEROTROPHIC NITRIFICATION AEROBIC DENITRIFIER WITH HIGH EFFICIENCY IN REMOVAL OF NITROGEN[J];Journal of Beijing Technology and Business University(Natural Science Edition);2010-02
4 LIANG Jing-nan,LIU Yi-wei*(Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China);The improvement for the phenomena of phosphate crystallization during making microbiological samples for scanning electron microscope observation[J];Journal of Chinese Electron Microscopy Society;2012-02
5 REN Yan-jun,QIN Su-ping(Analysis and Testing Centre of He Bei Normal University of Science&Technology,Qinhuangdao 066004,China);Study on Scanning Electron Microscope Working at Nonoptimum Observation Conditions[J];Analysis and Testing Technology and Instruments;2008-01
6 ZHANG Wei1,2,AI Qi-jun2,WU Zhen-yu1,2,WEI Yue-qin1,2(1Department of Plant Science and Technology,Beijing Agricultural College,Beijing 102206 China;2Department of Food Science,Beijing Agricultural College,Beijing 102206 China);Inhibitory effect of Pyrolin on Alternaria alternata causing occurrence of black spot in postharvest pear[J];Journal of Fruit Science;2008-05
7 WANG Wei1,XUE Xiao-ping1,YANG Hui1,XIE Qiong2(1.Faculty of Life Science,Northwestern Polytechnical University,Xi′an 710072,China;2.The Laboratory of Medical Supervises the Health Insurance,Chinese Astronaut Training Center,Beijing 100094,China);Study on Influence of Simulated Microgravity on Morphology and Surface Ultrastructure of Escherichia Coli[J];Chemistry & Bioengineering;2011-02
8 NI Hong1,XIONG Zhe1,ZHANG Shan1,ZENG Siquan1,LI Lin2(1.School of Life Science,Hubei University,Wuhan 430062;2.State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070);Effect of porous ceramic on the immobilized microorganisms and scanning electron microscopy[J];Journal of Hubei University(Natural Science);2011-02
9 WANG Liqun1,2,WANG Qing1,HE Yucai1,2,CAI Zhiqiang1,2,WANG Yun1,WANG Minghui1(1School of Pharmaceutical Engineering and Life Sciences,Changzhou University,Changzhou 213164,Jiangsu,China; 2Changzhou's Key Laboratory of Pharmaceutical Manufacture and Quality Control Engineering, Changzhou 213164,Jiangsu,China);A lipase-producing strain and its catalytic characteristics[J];Chemical Industry and Engineering Progress;2012-03
10 LIU Jian-nan,WANG Ping,YIN Ming-rui,WANG Lei College of Chemistry and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China;Isolation and Identification of a High-Efficiency Strain in the Removal of Nitrogen from MSG Wastewater Denitrification System and Study of Its Denitrification Characteristic[J];Research of Environmental Sciences;2010-03
【Secondary Citations】
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1 LI Xiang-Dang and LIU Jian Electron Microscopy Laboratory;A rapid method for the preparation of scanning electron microscopic specimens of erythrocyte[J];Journal of The Fourth Military Medical University;1992-05
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