Inhibitory effect of antisense VEGF_(121) and endostatin genes transfection on tumor growth and metastasis of human giant cell lung cancer

WU Xiao, ZHENG Jie, ZHU Jianjian,FU Jian, MA Chunshu, YOU Jiangfeng, CUI Xianglin, WANG Jieliang, FANG Weigang, ZHOU Airu, TANG Jian, WU Bingquan. Department of Pathology, Peking University, Beijing 100083, P.R.China  

Objective To explore the co operative inhibitory effect of antisense VEGF gene and endostatin gene transfection on tumor angiogenesis, tumor growth and metastasis of lung cancer. Methods Antisense VEGF 121 cDNA was transfected into PG cells(PG AS VEGF) by lipofectin. After PG AS VEGF cells were xenografted to nude mice, PsectagA endostatin gene was transfected into nude mice by electric pulse mediation. The MVDs in tumors and tumor biological characteristics were observed. Results ①The MVD in PG AS VEGF tumor in nude mice was significantly lower than that in PG vector tumor (PG AS VEGF and PG vector: 40.67±9.35 and 58.34±10.52, respectively) in nude mice. ②There was no significant difference between the PG vector tumor and PG AS VEGF tumor in early stage of the tumor growth in vivo. However, PG AS VEGF tumor grew significantly more slowly than PG vector tumor after 18 days (P0.05). ③PG AS VEGF tumor could lead to regional and/or distant lymph node metastases (16.7%, 2/12), which was much more infrequent than that in PG vector group (50%, 6/12). ④ PG AS VEGF tumor growth was remarkably inhibited by endostatin gene transfected at site of the tumor inoculation as compared with the control group in nude mice (P0.05). ⑤The PG AS VEGF tumors transfected with the endostatin gene at site of the tumor inoculation(AST) could also produce much lower regional and/or distant lymph node metastases rate (12.5%, 1/8) than that in the PG AS VEGF tumor transfected with the PsectagA vector (ASP)(75%, 6/8). Conclusion Endostatin gene transfection could cooperatively inhibit the growth and spontaneous lymph node metastasis of antisense VEGF gene transfected PG cells in nude mice.

【Key Words】： Lung neoplasms VEGF Endostatin Gene transfection
【CateGory Index】： R734.2

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