The clinical significance of detection of telomerase in bronchoalveolar lavage liquid in patients with lung cancer
JIAO Xianfa *, WANG Guobin, QIN Jianling, WANG Feng, WANG Ning. * Zhengzhou Fifth People Hospital, Zhengzhou, Henan 450003, P.R.China
Objective To investigate the diagnostic significance of detection of telomerase activity of bronchoalveolar lavage liquid (BALF) in primary lung cancer. Methods BALF of 63 patients with lung cancer and 31 patients with non cancerous pulmonary disease was collected, and telomerase activity was detected with silver stained TRAP. Brush cytology and BALF cytology were carried out simultaneously. Results The positive rate of telomerase was 76.2% (48/63) in lung cancer group, which was higher than 6.5% (2/31) in control group (χ 2=40.6, P0.01). The positive rate was also higher than that of brush cytology (58.7%, 37/63) (χ 2 =3.6, P0.05), and higher than that of BALF cytology (14.3%, 9/63) (χ 2=46.3, P0.01). In central type lung cancer group, the positive rate of telomerase was 71.4% (35/49), and higher than that of BALF cytology (12.2%, 6/49) (χ 2= 35.3 , P0.01), but had no significant difference compared to that of brush cytology (61.2%, 30/49) (χ 2=1.1, P0.05). In peripheral type lung cancer group, the positive rate of telomerase in BALF was 92.9% (13/14), and higher than that of brush cytology (50.0%, 7/14) and that of BALF cytology (21.4%, 3/14). Conclusion The telomerase activity could be detected in the BALF of the primary lung cancer. Hence, detection of telomerase in the BALF of patients may be helpful to diagnose lung cancer.