Study on anti-invasive effect and apoptosis induction of pentacyclic triterpenoid in human lung cancer cells

HUANG Wei, HUANG Jiqun, ZHANG Dongfang, ZHANG Ruiling, LIAO Zhaoquan. Department of Biochemistry, Guangzhou Medical College, Guangzhou, Guangdong 510182, P.R.China  

Objective To study the anti invasive effects and its mechanism and apoptosis induction of pentacyclic triterpenoid including glycyrrhizin (GL), 18β glycyrrhetinic acid (GA), ursolic acid (UA) and oleanolic acid (OA) in highly potentially metastatic lung cancer cell line (PGCL3). Methods The invasive ability, the adhesive ability, the migration ability and the activity of cathepsin B (CB) of PGCL3 cells treated with the four drugs were determined by the invasion test of reconstituted basement membrane, the laminin adhesion test, the chemotactic migration test and the enzymological method of CB. The apoptosis of the cells was detected with acridine orange ethidium bromide fluorescent stain (AO/EB) and TUNEL. Results The GL,GA, UA and OA could decrease the proliferative ability of PGCL3 cells, and their IC 50 values were 1.83 mmol/L, 145.3 μmol/L, 44.73 μmol/L and 40.71 μmol/L respectively. After treatment with 0.5 and 1.0 mmol/L GL, 25 and 50 μmol/L GA, 30 and 40 μmol/L UA, 35 and 45 μmol/L OA for 96 h, the invasive ability of the PGCL3 cells was significantly decreased compared with that of the control groups ( P 0.01 or P 0.001). The adhesive and migration ability, the secretion of CB and the colony formation number in semi solid agar were significantly decreased after PGCL3 cells were treated with the above concentration of the four drugs for 96 h ( P 0.05, P 0.01 or P 0.001), and the inhibition was in a dose dependent fashion. The percentages of apoptosis of the cells were obviously increased after treatment with the above concentration of the four durgs for 48 h, compared with the control group ( P 0.05 or P 0.01). Conclusion All of the four drugs can inhibit the proliferative and invasive ability, and induce apoptosis of the PGCL3 human lung cancer cells. The mechanism of anti invasion may be to inhibit the adhesion, migration, and the CB secretion of the cells.

【Key Words】： Lung neoplasms Glycyrrhizin 18β glycyrrhetinic acid Ursolic acid Oleanolic acid Invasion Apoptosis
【Fund】： 广州市科技攻关项目 (批准号 98 Z 0 4 1 0 1 )~~
【CateGory Index】： R734.2

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