Effect of Betametasone on NF - κB, IC AM -1 and IL - 6 Following Severe Acute Pancreatitis - associated Lung Injury in Rats
MO Jian,LIU Xin,HE Hai -yan,et al. (Department of Anesthesiology, Affiliated hospital of Guangdong medical college, Zhanjiang 524001, China)
Objective To study NF -κB activity, ICAM -1 and IL -6 level following severe acute pancreatitis -associated lung injury and the effect of betametasone on them in rats. Methods 36 SD rats were randomized into three groups each consisting of 12 rats:Sham -operated control group ( C group), severe acute pancreatitis group ( SAP group), betametasone group ( B group). SAP models were induced by retrograde injection of 5% sodium taurocholate(1 ml/kg) in the pancreatic duct. Group B were injected betametasone 10 ml/kg via vein after SAP models were made successfully. The activity of NF -κB and the level of ICAM -1 in lung were detected by immunohistochemical technique,and the level of IL -6 in serum and lung were measured by ELISA method. Results Histological change:There were some necrosis cells, more neu-trophiis infiltration, microvascular thrombus in both pancreas and lung tissue in SAP group, but less change in B group. The activity of NF -κB, the level of ICAM -1 and IL -6 in SAP group were significantly higher than those in C and B group(P 0.01) ,and the activity of NF -κB,the level of IL -6 of lung tissue in B group were higher than in C group (P 0.05). Conclusions Betametasone can inhibit the activity of NF -κB, decrease ICAM -1 and IL -6 level, alleviate severe acute pancreatitis -associated lung injury;animal model.
【CateGory Index】： R576