Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fudan University》 2002-01
Add to Favorite Get Latest Update

Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene ( Rc MDH ) and Its Expression in E.coli

JIANG Rui hua, LIN Chang fa, SHEN Guo an, QIAN Min, WANG Dong, DU Xi ling, HUANG Ji, YANG Jin shui (Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433,China)  
Using the maize MDH gene as probe, a novel rice full length cDNA was cloned through screening a rice seedling cDNA library. It showed 87% identity in nucleic acid sequence to ZHO 2(GenBank No.AF007581)of maize, and therefore was named as rice cytoplasmic malic dehydrogenase gene( RcMDH ). This gene encodes 322 amino acids, with putative molecular weight of 36.5 ku. The open reading frame of RcMDH was subcloned into reconstructed pGEX 4T 1 plasmid which expressed in E.coli with IPTG inducing. The MDH enzyme activity of its product was also certified.
【Fund】: 教育部重点资助项目
【CateGory Index】: Q789
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 HU Jian-Guan; ZHAO Xiang-Shan; LIU Jun; YUAN Zi-Qiang; YANG Jin-Shui (Institute of Genetics, Fudan University, Shanghai 200433);Isolation and Characterization of a cDNA Encoding Maize Cytosolic Malate Dehydrogenase[J];ACTA BOTANICA SINICA;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XU Dong-sheng et al(Xiaogan College,Xiaogan,Hubei 432000);Research on the Resistant Mechanism of the Rose Black Spot[J];Journal of Anhui Agricultural Sciences;2007-27
2 ZHAO Hao-qin et al(Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003 );Cloning and Sequence Analysis of Biological Clock Gene Bmtim2 in Bombyx mori L.[J];Journal of Anhui Agricultural Sciences;2008-17
3 ZHAO Wei,XU Wei-rong,XU Yan,HE Ming-yang,WANG Yue-jin(College of Horticulture,Northwest A & F University,Key Laboratory of Horticulture Plant Germplasm and Genetic Improvement of China Agricultural Ministry,Yangling,Shaanxi 712100,China);Cloning Full Length cDNA of Chinese Wild Vitis Novel Gene and Sequence Analysis[J];Acta Botanica Boreali-Occidentalia Sinica;2007-05
4 GUO Yu qi et al . (Dept. of Agronomy,Luoyang Agricultural College,Luoyang,471003);Gene Expression and the Mechanisms of Heterosis[J];Journal of Henan Vocation-technical Teachers College;2001-02
5 LENG Yue-qiang,HOU Xi-lin(National Key Laboratory of Crop Genetics & Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China);Changes in Protective Enzymes Activity in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino) Inoculated with Peronospora parasitica[J];Jiangsu Journal of Agricultural Sciences;2006-03
6 Wu Xuehong, Liu Xili, Liu Pengfei, Zhou Ligang, Wang Hongmei, Li Jianqiang (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094,China);Effect of 15% Hymexazol-Metalaxyl-Thiram seed coating formulation on the growth of watermelon seedlings and disease resistance-related enzymes[J];Journal of China Agricultural University;2003-03
7 ZHANG Zhuan XUE Wei WANG Xiang-Kun SUN Chuan-Qing**(Department of Plant Genetics and Breeding,State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Beijing Key Laboratory of Crop Genomics and Genetic Improvement,China Agricultural University,Beijing 100094,China);Studies on the Relationship between Heterosis and Gene Differential Expression Using Introgression Lines of Rice(Oryza sativa L.)[J];Journal of Agricultural Biotechnology;2005-06
8 FANG Feng,JIANG Xing-yin,JI Chun-tao,NIE Le-xing,WU Shuhua,ZHANG Peng(College of Plant Protection,Shandong Agricultural University,Tai′an 271018,Shandong Province,China);Effect of Seed Coating Agents on Growth and Disease Resistance Related Enzymes of Shannong Feed Corn No.7 Seedling[J];Chinese Journal of Pesticide Science;2009-01
9 ZHANG Yi-ping 1, 2, CHEN Xue-feng 1, XIONG Jian-hua 1, HUA Yang 1, LI Yang-sheng 1,* ( 1Key Laboratory of Ministry of Education for Developmen tal Biology, Wuhan University, Wuhan 430072, China; 2Medical Coll ege, Jiujiang University, Jiujiang 332000, China; );Isolation and Characterization of 26S Proteasome Subunit Gene OsHL1 Expressed Differentially Between Rice Hybrid and Its Parents[J];Chinese Journal of Rice Science;2004-06
10 MA Liu-feng, XIN Jian-hua, FU Zhen-qing (Department of Horticulture, Shihezi University, Shihezi, Xinjiang, 832003, China);Advances of study on disease resistance of melon[J];China Watermelon and Muskmelon;2005-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHU Li-ye~1, SHAO Hong-bo~2, LI Mao-yan(1.Chongqing University of Posts & Telecommunications, Chongqing 400065,P.R.China;2.State Key Laboratory of Soil Erosion and Dryland Farming, the Center of Soil and Water Conservation and Ecoenvironmental Research ,Chinese Academy of Sciences,Yangling 712100,P.R.China);DNA helicases in higher plants[J];Journal of Chongqing University of Posts and Telecommunications;2005-04
2 QIN Yan, XIA Xiuzhong (College of Agriculture, GuangXi University, Nannig 530005);Preliminary study on induction of callus in cassava[J];Guangxi Agricultural Science;2005-03
3 HUANG Jie,YAN Qingxiang,LI Kaimian,YE Jianqiu,XU Ruili(Institute of Tropical Crops Germplasm Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737);Techniques for rapid propagation of seed stock by Cassava shoot[J];Guangxi Agricultural Science;2005-06
4 HUANG Hua-cheng~1,TANG Guang-da~1,LUO Xiao-ying~1,ZHUANG Xue-ying~1,CHEN Yong-juan~2(1 College of Foresty,South China Agric.Univ.,Guangzhou 510642,China;2 Department of Applied Biology and Chemical Technology,Hong Kong Polytechnic University,Kowloon,Hong Kong);The effects of three Glomus fungi on growth of Manihot esculenta[J];Journal of South China Agricultural University;2005-04
5 Mo Rao 1 Wu Fanhua 1 Ye Jianqiu 2 Li Kaimian 2 (1 South China University of Tropical Agriculture, Danzhou, Hainan, 571737; 2 Tropical Field crops and Pasture Institute,CATAS,Danzhou,Hainan,571737);Cassava in Vitro Culture[J];Journal of South China University of Tropical Agriculture;2003-01
6 Zou Jixin1 Li Kaimian1 Wang Wenquan2 (1 Institute of Tropical Crop Germplasm Resources, CATAS, Danzhou, 571737; 2 Institute of Tropical Bioscience, State Key laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 571101);Evaluation on Microsatellite Molecular Genetic Diversity of Cassava Germplasm Resources by SSR Marker[J];Journal of South China University of Tropical Agriculture;2005-02
7 SUN Liang xian,XIE Jin jin,HUANG Zhou ying ( Department of Biology,Quanzhou Normal College,Fujian 362000, China);Advances in the Study of Expressed Sequence Tags[J];Journal of Quanzhou Normal College;2002-04
8 Li Kaimian Lin Xiong Ye Jianqiu Huang Jie Xu Ruili Zhang Weite(Tropical Field Crops and Animal Husbandry Research Institute,CATAS,Danzhou,Hainan571737);Selection and Breeding of Manihot esculenta Crantz.cv.M.SC6[J];Chinese Journal of Tropical Crops;2002-04
9 LI Yong-qing, ZENG Wei-qi, ZHU Chuan-bing, PI Hua-liang,DAI Qi, WU Xiu-shan [WT5”BX](College of Life Sciences,Hunan Normal University, Changsha 410081, Hunan, China);Primary Studies on a Novel Human ZNF322 Gene[J];Life Science Research;2001-02
10 XU Jian-Miao ZHENG Yu-Guo** SHENG Yin-Chu (Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 31001 4);The Nitrilase: Sources, Structure, Mechanism and Applications*[J];Microbiology;2005-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Jiajun;Li Xing;Hou Mingshen(Huazhong Agricultural University, Wuhan 430070);THE CHARACIERISTICS OF WHEAT VARIETIE'S RESISTANCE TO WHEAT SPINDLE STREAK MOSAIC VIRUS (WSSMV) Ⅱ.RELATIONSHIP BETWEEN THE RESISTANCE TO THE DISEASE(WSSMV) AND THE PHYSIO-BIOCHEMISTRY OF WHEAT VARIETIES[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1996-05
2 Lu Chengqun (Department of Forestry, Forestry Branch of Guangxi Agricultural College);CHANGES OF PEROXIDASE ISOENZYMES IN MANGO SEEDLING INFECTED BY COLLETOTRICHUM GLOEOSPORIOIDES PENZING[J];Chinese Journal of Tropical Crops;1991-02
3 Li Baoju Li Fengyun (Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161);Changes in Activities and Eletrophretic Patterns of Peroxidase and Polyphenoloxidase in Cucumbers During Infection with Cladosporium cucumerinum[J];SCIENTIA AGRICULTURA SINICA;1998-01
4 Ye Huazhi Wu Guangqing(Sichuan Agricultural University,Ya-an,Sichuan);CHANGES IN ACTIVITIES OF PEROXIDASE AND ESTERASE IN WHEAT HEADS INFECTED BY FUSARIUM GRAMINEARUM[J];Acta Phytopathologica Sinica;1988-03
5 Wu Chunren Wang Quanmao(Dept.of Plant Protection,Huazhong Agri.Uni.);BIOCHEMICAL STUDIES ON RESISTANCE TO FUSARIUM MONILIFORME SHELD IN CORN[J];Acta Phytopathologica Sinica;1988-04
6 Fan Zihong;Shen Ruixiang;Zhou Zhongming Beijing Forestry University;THE RELATIONSHIP BETWEEN THE ACTIVITIES OF PEROXIDASE AND PHYNELALANINE AMMONIA LYASE AND THE RESISTANCE OF POPLAR TO LEAF RUST[J];Acta Phytopathologica Sinica;1989-02
7 Xie Weilong Leng Huaiqiong(Sichuan Agricultural University);THE PEROXIDASE ISOENZYME OF APPLE TREES INFECTED BY MARSSONINA CORONARIA[J];Acta Phytopathologica Sinica;1989-04
8 Tian Xiuming Du Lifeng (Dept. of Plant Protection, Shanxi Agri. Univ.);THE RELATIONS BETWEEN RESISTANCE OF COTTON TO VERTICILLIUM AND FUSARIUM WILT AND PEROXIDASE ACTIVITV[J];Acta Phytopathologica Sinica;1991-02
9 Li Jing Li Rongqian Yuan Wenjing (Department of Biology, Wuhan University, Wuhan);ON THE CHANGE OF ENZYME ACTIVITES OF CUCUMBER LEAF INFECTED BY PSEUDOPERONOSPORA CUBENSIS(BERK.ET CTRT) ROSWS.[J];Acta Phytopathologica Sinica;1991-04
10 Guo Wenshuo (Department of Forestry, Fujian Forestry College, Fujian Nanping 353001);ON MECHANISM OF TUNG OIL TREE VARIETIES RESISTING BLACK SPOT (Mycos phaereila aleuritidis Hemsi) AND RELATION TO SUPEROXIDE DISMUTASE[J];Acta Phytopathologica Sinica;1994-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved