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《Journal of Fudan University》 2002-06
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A Preliminary Study on the Molecular and Chemical Characteristics of Authenticity (Daodixing)of a Traditional Chinese Medicinal Plant-Forsythia suspense Vahl

GU Hua1,GAI Ling1,ZHOU Tongshui2,WU Xianli1,JIANg Keji1,CHEN Jiakuan2,3,KUAI Benke1,3(1.Department of Biochemistry,School of Life Sciences,Fudan University; 2.Institute of Biodiversity Science,FudanUniversity; 3.Research Center of Gene Diversity and Designed Agriculture,Fudan University,Shanghai 200433, China)  
To address the authenticity (daodixing, a concept used to judge the quality of Traditional Chinese Medicines (TCM) mainly based on the growing environments as well as the varieties of medicinal plants) of a Traditional Chinese Medicinal plant, Forsythia suspensa, the genetic relationship of 11 cultivars from sixdifferent regions of Shanxi province (Licheng,Changzhi, Tunliu, Huguan, Pingshun, Anze) was analyzed using the randomly amplified polymorphic DNAs (RAPD) technique. The contents of forsythin, an indicative component of Forsythia suspensa, in these cultivars were analyzed using high performance liquid chromatography(HPLC). The RAPD analysis showed that there was a significant genetic diversity among the 11 cultivars. These cultivars were arranged into three genetic branches (A, B and C) using the Philip3.6 software and Wagner′s method. The branch A containing the only cultivar from Huguan was genetically distant from the other two branches, B and C, which were relatively close. The HPLC analysis showed that there was also a great variation in the contents of forsythin among these cultivars. Most dramatic variations were found between the branchA (8.7 mg/g) and the branchB or the branchC. However no significant variations were found within the three branches, nor between the branchB (the mean 0.53mg/g)and the branchC (the mean 0.67mg/g). These results suggest that the formation and accumulation of forsythin could be affected substantially by the genetic basis of Forsythia suspense. It is particularly true in this case since these cultivars were collected from relatively less diverse environments. This work has shown some promise that the authenticity of Traditional Chinese Medicinal plants can be addressed using RAPD and HPLC techniques.
【Fund】: 上海市中药重点基础研究项目:双黄连及银杏叶制剂原料药材(金银花、黄芩、连翘、银杏)道地性和GAP生产相关问题研究
【CateGory Index】: R282.71
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