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《Journal of Fudan University》 2005-04
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Cloning and Sequencing of a Novel Avirulent Gene from Xanthomonas oryzae pv.Oryzae

LIANG Bin~(1,2),ZHANG Xin-jun~1,YANG Chen~1,GUO Bin~1,DAI Lu-yuan~2,YU Teng-qiong~2,SHEN Da-leng~1(1.Institute of Genetics,School of Life Sciences,Fudan University,Shanghai 200433,China;2.Crop Germplasm Resources Station,Yunnan Acadmy of Agriculture Sciences,Kunming 650205,China)  
A novel avirulent gene was cloned from Xanthomonas oryzae pv.oryzae PXO339 strains,which is the standard representative strain for race 9a in the Philippines,The full-length gene spans 2 118 bp and encodes a protein of 705 amino acids.Its molecular weight and isolectric point are 74.7 ku and 6.585,respectively.BLAST search in NCBI indicated that the gene belongs to avrBs3 gene family,and designated as arp3.Alignment Arp3 with others AvrBs3-like proteins uncovered that the 5' terminal of arp3 lacks of two segments,whose sizes are 144 and 45 bp,respectively.The 5' character of arp3 is common in all Xoo strain genomic DNA.The central region of the arp3 contains 5.5 copies of 102 bp repeats,the smallest copy number of repeats found in avrBs3 gene family by now.Three functional nuclear localization signals (NLSs) and an acidic activation domain (AAD) are also found in the C-terminal region of Arp3.
【Fund】: Mcknight基金(14001404);; 云南省自然科学基金重点资助项目(200C0010Z)
【CateGory Index】: S435.111.47
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