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《Journal of Fudan University(Natural Science)》 2006-06
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Callus Induction and Darkening Inhibition in Tissue Culture of Taxus chinensis var.mairei and Taxus media

LI Li,ZHANG Yan-fan,HE Kang,FAN Zheng-qiu,WANG Xiang-rong(Department of Environmental Science and Engineering,Fudan University,Shanghai 200433,China)  
Callus induction and darkening inhibition were studied in tissue culture of Taxus chinensis var.mairei and Taxus media based on Uniform Design.Results indicated that caudex is more effective in callus induction than leaf and a linear regression relationship exists between callus induction ratio and its diameter for a certain plant.Culture medium B_5 with the addition of 6-BA 0.3 mg·L~(-1) and NAA 2.9 mg·L~(-1)(T.media),6-BA 0.1 mg·L~(-1) and NAA 1.7 mg·L~(-1)(T.chinensis var.mairei) gave optimum cell induction and growth.The caudex and leaf of T.chinensis var.mairei induced callus earlier than that of T.media on the average.The average callus diameter of caudex and leaf of T.chinensis var.mairei was bigger than that of T.media.An addition of vitamin C 0.01—0.2 mg·L~(-1) in culture medium was effective in callus darkening inhibition without significant influence on cell growth.
【Fund】: “十五”’211工程”重点建设项目(生物多样性与区域生态安全)
【CateGory Index】: S791.49
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