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Single-oocyst Isolation and Species Identification of Eimeria in Chicken

WANG Xin,HUANG Bing,ZHAO Qi-ping,HAN Jing-fang,HAN Hong-yu,DONG Hui,JIANG Lian-lian (Key Laboratory for Animal Parasitology of Ministry of Agriculture,Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,China)  
To identity the strains of Eimeria conserved in our laboratory are purebred,11 strains of 6 Eimeria species were separated by single-oocyst method and infected chicken of 1—5 days old.179 chicken were inoculated and 42 of them were infected successfully.The rate of successful is 23.46%.Then the 11 of 42 isolated-strains were identified according to the location of coccidia in the intestine,prepatent period,size and shape of oocysts,shape index(length divided width),size and shape of sporocysts,the shortest sporulation time.1 strain of E.acervulina,2 of E.tenella,6 of E.maxima,1 of E.mivati and 1 of E.necatrix are identified,respectively.These results indicated that the E.brunetti Guangdong strain conserved in our laboratory was polluted by E.tenella.
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