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《Journal of Fudan University(Natural Science)》 2007-06
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Study on Cytochemical Location of Acid Phosphatase and Alkaline Phosphatase of Litopenaeus vannamei

SUN Jing-qiu1,XU Yan1,ZHANG Hui-qi1,YE Jun-qiang2 (1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China;2.Shanghai Fishery Technical Extension Center,Shanghai 201400,China)  
The activities and location of acid phosphatase(ACP) and alkaline phosphatase(AKP) in the liver(L),muscle(M),heart(H) and eye(E) of Litopenaeus vannamei were studied with enzyme cytochemical technique of electronic microscope,compared it with the infected shrimp.The results showed that ACP positive reactions were mainly deposited in lysosome,nucellar cell,cellular endomembrane,mitochondrium,and endoplasmic reticulum in four tissues of the healthy shrimp.Furthermore,ACP activity was located in microvilli and around partly lipid droplet in liver.AKP positive reactions were mainly deposited in cell membrane,nucellar cell,lysosome,endoplasmic reticulum and cellular endomembrane in four tissues.There were also AKP positive granules in microvilli and around lipid droplet in liver.In the infected shrimp,the obviously histopathological changes were detected in four tissues,and more myelin figures appeared in the cell of each tissue.There were evident differences in tissues between diseased shrimp and healthy shrimp in the activities and location of ACP and AKP.The activities of ACP and AKP in corresponding organelles of the infected shrimp decreased or disappeared.At the same time,the stronger ACP and AKP positive reaction granules were observed in cytoplasm of most tissues and myelin figures.
【Fund】: 上海市教委高校科技发展基金项目(No.05DZ30)
【CateGory Index】: S945
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