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《Journal of Fudan University(Natural Science)》 2010-01
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Comparison of Cultured Microbial Diversity in Chongming Dongtan Soils Covered by Different Plants

MENG Han1,HUI Wei2,XIAO Yi-ping1,TIAN Guan-rong2,QUAN Zhe-xue1 (1.Department of Microbiology and Microbial Engineering,School of Life Sciences,Fudan University Shanghai 200433,China;2.College of Science,Yanbian University,Yanji 132300,China)  
Chongming Dongtan is one of the main wetlands in China.Here,three soil samples from tidal flat,Scirpus triqueter root region and Phragmites australis root region were cultivated by diluted R2A medium.Through plate counting method,the bacterial numbers in one gram soil were 8.6×106 cfu in tidal flat sample,1.3×107 cfu in Scirpus triqueter sample and 1.05×108 cfu in Phragmites australis sample.According to the results of 16S rRNA gene sequences of isolated 84 strains,the diversity index of the tidal flat was the highest.Comparing with 16S rRNA gene sequences of standard strains and the constructed phylogenetic tree,the taxonomy of these strains was defined.These isolates belong to 30 genera of γ-Proteobacteria,Bacteroidetes,Firmicutes,α-Proteobacteria,and Actinobacteria.And up to 8 genera related bacteria were only isolated from tidal flat sample.
【Fund】: 国家科技支撑计划“长三角村镇土地规划利用技术开发”资助项目(2006BAJ05A11)
【CateGory Index】: S154.3
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