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《Feed Industry》 2013-07
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Determination of tryptophan in corn gluten meal and creep feed by HPLC method

Song Jiying  
The experiment was conducted to establish a HPLC for determination of tryptophan.Tryptophan was determinated on HPLC instrument fixed a Agilent C18 column(4.6 mm×150 mm,5 μm) with UV detection at 280 nm,and the mobile phase was 0.008 5 mol/l sodium acetate solution+MeOH=95+5,the flow rate was 1.0 ml/min.The calibration curve was linear(r=0.999 9) within the range of 4.656~298 μg/ml for tryptophan,and LOD was 0.033 5 μg/ml,levels of tryptophan in the two feed were in reasonable range.This method was simple,quick,accurate and effective for determination of tryptophan.It was suitable for the quality control for tryptophan.
【CateGory Index】: S828.5
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