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《Chinese Journal of Luminescence》 2011-01
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Luminescence Spectroscopy of Conjugation Reaction between Bovine Serum Albumin and Spectroscopic Probe Ponceau S

LIU Bao-sheng,XUE Chun-li,WANG Jing,YANG Chao,LU Yun-kai(Key Laboratory of Medical Chemistry and Molecular Diagnosis,Ministry of Education,Center of Physics and Chemistry,Hebei University,Baoding 071002,China)  
Ponceau S(PS) can quench the fluorescence of bovine serum albumin(BSA) in the aqueous solution of pH=7.40.The static fluorescence quenching process between BSA and PS was confirmed and the binding constant,the number of binding sites and thermodynamic parameters between BSA and PS were obtained.It showed that the number of binding sites was 1 and the electrostatic attraction played an important role in the binding of BSA to PS.Based on the theory of Frester energy transfer,the binding distance(r7.0 nm) between PS and BSA was obtained.Studies utilizing synchronous spectra showed that the conjugation reaction between PS and BSA would affect the conformation of BSA,leading to the weak polarity around tyrosine residues and the strong hydrophobicity.The site markers competitive experiments indicated that the binding of PS to BSA primarily took place in sub-domain ⅡA(site Ⅰ).
【Fund】: Project supported by National Science Foundation of China(20675024)~~
【CateGory Index】: O482.31
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Bing-wei1,LI Bo1,2,XIA Wen-shui2,DING Li1(1.Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China;2.State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China);Selection of Models in the Calculation of Binding Constants in the Study of Interaction between Molecules by Fluorescence Spectroscopy[J];Progress in Pharmaceutical Sciences;2011-07
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Yu-fang1,2,LI Jian-qing1,2,XU Zhi-cheng1,WEI Yan-li1,SHUANG Shao-min1,DONG Chuan1 1.Department of Chemistry,Shanxi University,Taiyuan 030006,China2.Department of Chemistry,Jinzhong University,Yuci 030600,China;Study on the Interaction between ICT Fluorescence Probe and Bovine Serum Albumins[J];Spectroscopy and Spectral Analysis;2008-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 PENG Fang-yi,YANG Hai-yan,JIANG Hai-rong,XU Jian-jian(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China);Application of Immunoassay Analysis in Doping Test of Pesticide Residues[J];Journal of Chongqing University of Technology(Natural Science);2011-06
2 XIE Ying,LI Yong-cheng,YANG Li-yun,ZHANG Ye-zhong (College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China);Investigation on Interaction of Tegafur with Bovine Serum Albumin[J];Chemistry & Bioengineering;2010-09
3 GAO Hong~(1,*) MA Min-quan~2 ZHOU Lei~3 JIA Run-ping~3 CHEN Xing-guo~3 HU Zhi-de~3 1.Key Laboratory of Western China's Environmental Systems(Ministry of Education),College of Earth and Environment Sciences,Lanzhou University,Lanzhou 730000,China. 2.Lanzhou Environmental Monitoring Station,Lanzhou 730000,China 3.Department of Chemistry,Lanzhou University,Lanzhou 730000,China;Interaction of DNA with aromatic hydrocarbons fraction in atmospheric particulates of Xigu District of Lanzhou,China[J];环境科学学报(英文版);2007-08
4 Wu,Zhisheng Zhang,Jing Chen,Wang Hu,Juan (Department of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350108) (Key Laboratory of Chinese Pharmacies for Fujian Higher Education,Fuzhou 350108);Studies on the Aggregation and Interaction of Daidzin with Bovine Serum Albumin by Fluorescence Spectrum and Laser Light Scattering[J];Acta Chimica Sinica;2009-14
5 CAO Tuan-wu﹡,YANG Ji-dong,WU Xing-fa,YI Xing-ming(Department of Chemistry and Environment,Yangtze Normal University,Chongqing 408003,China);Spectrophotometric study on the interaction between ranitidine and bovine serum album[J];Chemical Research and Application;2009-09
6 FAN Cheng-ping, SHANG Zhi-cai, GUO Ming, YU Qing-sen (Department of Chemistry, Zhejiang University, Hangzhou 310027, China);Study on the interaction between gatifloxacin and bovine lactoferrin by fluorescence spectrophotometry.[J];Journal of Zhejiang University(Sciences Edition);2005-01
7 HE Wenying1,2, YAO Xiaojun1, LIU Pengjun2, GAO Zhenxia2 & HU Zhide1 1. Department of Chemistry, Lanzhou University, Lanzhou 730000, China; 2. Department of Chemistry, Hainan Normal University, Hainan 571158, China;Molecular modeling and spectroscopic studies on the binding of guaiacol to human immunoglobulin[J];中国科学(B辑:化学)(英文版);2006-06
8 WANG Yang,CHEN Yan-ping,ZHANG Ye-zhong(College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China);Investigation on the Interaction of 2,4-Dichlorophenol with Human Serum Albumin[J];Chemistry & Bioengineering;2011-05
9 ZHANG Jing,XIANG Xun,FANG Ting,Chen Yan-ping,ZHANG Ye-zhong(College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China);Spectroscopic studies on the interaction of indomethacin with bovine serum albumin[J];Chemical Research and Application;2011-07
10 ZHAO Ling-zi,CHI He,TENG Hong-hui,SHI Shu-yun(College of Environmental Engineering,Jilin Normal University,Jilin Siping 136000,China);Study on Toxic Effect of Methyl Violet on Bovine Serum Albumin by Spectroscopy Method[J];Liaoning Chemical Industry;2011-12
China Proceedings of conference Full-text Database 1 Hits
1 X.M.Cao L.Liao L.M.Du~* Analytical and Testing Center,Shanxi Normal University,Shanxi linfen,041004,China;Fluorescence Study on the Interaction of Sulfamethoxazole and Bovine Serum Albumin[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Bao-sheng,XUE Chun-li,WANG Jing,YANG Chao,LU Yun-kai (Key Laboratory of Medical Chemistry and Molecular Diagnosis,Ministry of Education,Center of Physics and Chemistry,Hebei University,Baoding 071002,China);Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods[J];Chinese Journal of Luminescence;2010-02
2 Ci Yunxiang~*; Chang Wenbao; Tie Jianke(Department of Chemistry, Peking University, Beijing 100871);Application of Chemiluminescence in Nucleic Acid Hybridization Analysis[J];Chinese Journal of Analytical Chemistry;1992-09
3 Feng Xizeng, Bai Chunli, Lin Zhang, Wang Naixin, Wang Chen (Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080);The Interaction between Acridine Orange and Bovine Serum Albumin[J];Chinese Journal of Analytieal Chemistry;1998-02
4 Xu Yan; Huang Hanguo (Department of Chemical Engineering, Hebei Institute of Science and Technology, Tangshan 063009) Shen Hanxi (Department of Chemistry, Nankai University, Tianjin 300071);Fluorescence Quenching Behavior of Human Serum Albumin by Quinolone Antibacterials[J];Chinese Journal of Analytieal Chemistry;1998-12
5 Li Yuanfang; Huang Chengzhi; Hu Xiaoli (Institute of Environmental Chemistry, Southwest Nounal University, Chongqing 400715);The Principles and the Applications of Resonance Light Scattering Technique in the Research of Biochemistry and Bioanalysis[J];Chinese Journal of Analytieal Chemistry;1998-12
6 Can Qiu'e1, Li Zubi2, Wang Jialin2, Li Chongning2 (Department of Chemistry1 , Adult Education College2, Yunnan University, Kunming 650091);Spectrophotometric Study on the Interaction of Azocarmine B with Serum Albumin and Its Analytical Applications[J];Chinese Journal of Analytieal Chemistry;2002-02
7 Li Shuwei, Li Na, Zhao Fenglin , Li Kean (College of Chemistry and Molecular Engineering, Peking University, Beijing 100871);Determination of Protein with Eriochrome Black T as a Probe of Resonance Light Scattering[J];Chinese Journal of Analytieal Chemistry;2002-06
8 Zhou Qiuyun, Yu Ying  (Department of Chemistry, South China Normal University, Guangzhou 510631);Interaction Studies of 2-(8-Hydroxyquinoline-5-sulfoacid-7-azo)-1,8-dihydroxy -3,6-naphthalene disulfoacid with Bovine Serum Albumin[J];Chinese Journal of Analytical Chemistry;2003-08
9 Liu Yongming *, Li Guizhi, Sun Xifen (College of Chemistry, Yantai University, Yantai 264005);Study on the Interaction between Colchicine and Bovine Serum Albumins by Fluorescence Method[J];Chinese Journal of Analytical Chemistry;2004-05
10 Xu Wenxiang, Pang Yuehong, Shuang Shaomin*(School of Chemistry and Chemical Engineering, Key Laboratory of Chemical Biology and Molecular Engineering,Shanxi University, Taiyuan 030006);Fluorescence Study on the Interaction of Human Serum Albumin with Hydrochlorothiazide[J];Chinese Journal of Analytical Chemistry;2004-12
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 YAN Cheng-nong1, SHANGGUAN Yun-feng1,TONG Jin-qiang1,LIU Yi2,PANG Dai-wen2,PAN Zu-ting2 and QU Song-sheng 1.Department of Chemistry, The Jinzhou Normal University, Jinzhou 434020,China 2.The School of Chemistry and molecular science, Wuhan University, Wuhan 430072,China;Study on Thermodynamics of Binding Reaction of Dipyridamole with Bovine Serum Albumin[J];Spectroscopy and Spectral Analysis;2003-03
2 BIAN Wei1, WEI Yan-li2, WANG Ya-ping2, DONG Chuan 2 1. Medical University of Shanxi, Taiyuan 030001, China 2. Chemistry and Chemical Engineering College, Shanxi University, Taiyuan 030006, China;Study on Interaction of Caffeine and Theophylline with Bovine Serum Albumins[J];Spectroscopy and Spectral Analysis;2006-03
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