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《Journal of Chongqing University(Natural Science Edition)》 2004-08
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Cloning of aspergillus niger F246 phytase gene and its a new type of expression vector construction

HUO Dan-qun~(1), FAN Shou-cheng~(1,2), ZHANG Yun-ru~(1), HOU Chang-jun~(1), FAN Shou-jun~(2) gineering Chongqing University, Ministry of Education, Chongqing University, Chongqing 400030, China; 2.Chongqing Pig Breeding Science Research Institute,Fan Shoucheng, Chongqing 402460, China)  
This research amplified the phytase gene of Aspergillus niger F246 by the polymerase chain reaction(PCR), which is selected and identified by ourselves.Then the product of PCR was inserted respectively into the lactobacillus expression vector pMG36e to construct the new type of expression vector pMG36e-phyA. The genomine DNA of the Aspergillus niger F246 strain is used as the template for PCR,and the PCR product was subcloned into pMD18T. After sequncing the coding region and analying the sequnce of amino acids, the product of PCR was cutted from pMD18T and subcloned into pMG36e for the engineering strain of Lactobacillus MG1363-pMG36e-phyA. The construction of new recombinant expression vector pMG36e-phyA for the phyA gene lays the base of the study on obtaining large and high active phytase and developing the new microbial ecologicalagent.
【CateGory Index】： Q785
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【References】
Chinese Journal Full-text Database 3 Hits
1 FU Nian,LU Fanggen,YANG Xuefeng1Department of Gastroenterology,the Second Xiangya Hospital,Central South University,Changsha,410011,China2 Department of Gastroenterology,Nanhua Hospital,Nanhua University,Hengyang,Hunan,421002,China;Construction of Prokaryotic Expression Plasmid of AdipoQ Gene of Rat and Its Expression in Lactobacillus Lactis[J];Journal of Medical Molecular Biology;2008-01
2 WEN Ya-nan,WANG Xue-ying,XIA Run-xi,WANG Yuan-yuan(College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China);Cloning of a phyA from Aspergullus niger and Its over Expression in Escherichia coli[J];Journal of Shenyang Agricultural University;2009-03
3 YANG Hong-lan1,2,WANG Wei2,3,BAO Hui-fang2,CUI Chun-sheng2,Shanqimuge1,2,HU Shuang1,2 (1.Key Laboratory of Agricultural Technology,Shihhotze University, Urumqi 832003,China;2.Insititute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;3.Xinjiang Research Center of Microbial Engineering of Special Envronmental,Urumqi 830091,China);Environmental Preliminary Report of Research on Expression System in a Kind of Candida Utilis[J];Xinjiang Agricultural Sciences;2008-03
【Citations】
Chinese Journal Full-text Database 7 Hits
1 XIA Li qiu,CHENG Hai na,CHEN Yu,MO Xiang tao (College of Life Science,Hunan Normal University,Changsha,410081 Hunan,China);Phytase and Adances in Its Gene Engineering[J];Acta Laser Biology Sinica;2001-03
2 LI Hong-jian 1, CHEN Zhen-qiu 1, ZHANG Yi 2 PAN Dong-mei 1, HE Ming-rui 1, ZHANG Jun-hui 2 (1. Dept. of Biotechnology, Jinan University, Guangzhou 510632, China; 2. Dept. of Biotechnology, South China University of Technology, Guangzhou 51;Studies on the expression of Aspergillus niger phytase gene in Escherichia coli JM-109-pTrcHis2A-phyA[J];Journal of Jinan University(Natural Science & Medicine Edition);2001-05
3 Yao Bin Zhang Chunyi Wang Jianghua Fan YunliuShi xiuyun wang Yaru Li shuming(1Feed Research Institute,Chinese Academy of Agricultural Scices,Beijing 100081;2 Biotechnology Research Center, Chinese Academy of Agricultural Sciences,Beijing 100081);Islation of Aspergillus niger 963 withPhytase Activity and Cloning Its Phytase Gene phy A2[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1998-01
4 Jia Shifang\ Wang Yinyu\ Guo Xinhua\ Huan Liandong (Institute of Microbiology,The Chinese Academy of Sciences,Beijing 100080);The Factors Affected Transformation Efficiency of Lactobacillus by Electroporation[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1998-04
5 XiangHua XhangYiqing WeiWenzhong TanHuarong (InstituteofMicrobiology ,ChineseAcademyofSciences,Beijing 1 0 0 0 80 );EXPRESSION AND ACTIVITY ANALYSIS OF THE HUMAN GLUTATHIONE-S-TRANSFERASEINLACTOCOCCUSLACTIS[J];ACTA MICROBIOLOGICA SINICA;2000-02
6 Wang Hongning 1\ Wu Qi 2\ Liu Shigui 1\ Xie Jing 2\ Ma Menggen 2 ( 1College of Life Science,Sichuan University,Chengdu,610064China) ( 2College of Animal Technology and Science,Sichuan Agricultural University,Ya'an 625014,China);CLONING AND SEQUENCE ANALYSIS OF THE PHYTASE PHYA GENE OF ASPERGILLUS NIGER N25 [J];Acta Microbiologica Sinica;2001-03
7 Xiao-ying,ZHANG Chao-wu,PEI Xiao-fang,et al (West China School of Public Health,Sichuan University,Chengdu 610041 ,China);A STUDY ON MAIN CHARACTER OF THREE LACTIC ACID BACTERIA AS VECTOR-HOST SYSTEM[J];Chinese Journal of Microecology;2001-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Wen-hui et al(Key Laboratory of the Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122);Constitutive Expression of the Phytase Gene from Thermomyces sp.in Pichia pastoris[J];Journal of Anhui Agricultural Sciences;2008-22
2 TIAN Zhi-huan1,SHEN Bao-zhong2(1.Department of Biology,Dezhou University,Dezhou 253023,China;2.Department of Life Science,Binzhou University,Binzhou 256603,China);Effects on GSTs Activity of Loach by Water Polluted with Waste Cells[J];Journal of Binzhou University;2007-03
3 KANG Ping,WANG Qiu-kuan(Key and Open Laboratory of Aquatic Product Processing and Utilization of Liaoning Province,Department of Food Engineering,Dalian Fisheries University,Dalian 116023,China);Enzymes Extracted from Abalone Viscera and Its Potential Application[J];Fisheries Science;2006-03
4 Yi Yongzhu 1 Chen Yin 1 Zhang Zhifang 1 He Jialu 1 Qin Jian 2( 1Key Laboratory of Silkworm Biotechnology,Ministry of Agriculture,Sericultural Research Institute, Chinese Academy of Agricultural Sciences,Zhenjiang 212018; 2Sichuan Huashen Company,Chengdu 610075);Construction of the Host Range Expanded Recombinant-Rescure Inscet Baculovirus Expression Vector and the Expression of Foreign Gene[J];Acta Sericologica Sinica;2002-04
5 ZHAO Zhen-ao,XU Xiu,DENG Kai-bo,TANG Li-jie,LI Yi-jing ( Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC );Feasibility study of lactic acid bacteria as host system[J];Journal of Northeast Agricultural University;2005-04
6 Jiang Zhiguo Wang Shizhang He Feiyan (Animal science technology college,Guangxi University Nanning 530005);Studies and Developments on Phytase and Its Appliance in Animal Feed[J];Journal of Guangxi Agriculture;2005-04
7 WANG Jun-mei~(1,2),SONG Wei-ping~2,ZHANG Li~2 (1.College of Life Science of Capital Normal University,Beijing 100037,China;2.Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China);Cloning of Phytase phy A from Aspergillus usamii 2418[J];Acta Agriculturae Boreali-Sinica;2006-03
8 KE Xiao-jing,GUO Run-fang,YU Hong-wei,JIA Ying-min (College of Food Science,Agriculture University of Hebei,Baoding 071001,China);Cloning and Sequence Analysis of the PhyB Gene of Aspergillus niger WP1[J];Acta Agriculturae Boreali-Sinica;2007-02
9 LEI Zheng-yu,HE Li-hua,LI Zhang-wu(Hubei Vocational College of Ecological Engineering,Wuhan 430200,China);On Exogenous Gene Expression in Prokaryotes and Eukaryotes[J];Journal of Hubei Vocational College of Ecological Engineering;2008-03
10 NI Hong-bo,QU Jin,SHI Xing-ming et al.;The New Evolve of Phytase in Characteristic of Molecular Biology and Application Foreground[J];Journal of Heilongjiang August First Land Reclamation University;2005-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
2 LIU Yong hai, LI Da li *, YANG Cheng li, SHI Ruo fu, WANG Shi ming, LU Lu de (Department of Biology Engineering, Nanjing University of Science and Technology, Nanjing 210094, China);Screening of phytase producing strain and purification of phytase and its property[J];Industrial Microbiology;2004-02
3 ZHANG Xue-wen1,2,TIAN Zhi-jian1,WU Yong-yao1,ZHOU Shuang-de2,KANG Dong-li1(1.College of Bioscience and Technology,HNAU,Changsha 410128,China;2.Institute of Agricultural Bio-Engineering,Changsha 410128,China);The Cloning and Expression of β-Mannanase Gene in E. coli[J];Journal of Hunan Agricultural University;2005-06
4 XIA Li qiu,CHENG Hai na,CHEN Yu,MO Xiang tao (College of Life Science,Hunan Normal University,Changsha,410081 Hunan,China);Phytase and Adances in Its Gene Engineering[J];Acta Laser Biology Sinica;2001-03
5 PAN Dong-mei, LI Hong-jian, LI Bai-hua, FANG Ling, CHEN Zhen-qiu, CHEN Hao-jun (Dept. of Biotechnology, Jinan University, Guangzhou 510632, China);Production of phytase by ferment of Aspergillus ficuum and kinetic characterization of phytase[J];Journal of Jinan University(Natural Science & Medicine Edition);2001-05
6 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
7 WANG Hong-Ning1,2 MA Meng-Gen1 WU Qi1 LIU Shi-Gui2 LUO Yan1 (1College of Animal Technology & Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014) (2 College of Life Science, Sichuan University, Chengdu, Sichuan, 610064);OVER-EXPRESSION RESEARCH OF ASPERGILLUS NIGER N25 PHYTASE GENE (PHYA) IN PICHIA PASTORIS[J];Mycosystema;2001-04
8 ZHENG Guang-yu(College of Life Sciences, Beijing Normal University, Beijing 100875 );Research Progress on Eucariotic Expression System[J];Journal of Kashgar Teachers College;2004-06
9 Sachurfu 1, LUO Liao-fu 2(1.Department of Physics,Inner Mongolia Normal University; 2.Department of Physics,Inner Mongolia University,Huhhot 010022,China);A SEQUENTIAL MODEL ON PROTEIN DENATURATION[J];Journal of Inner Mongolia Normal University(Natural Science Edition);2002-01
10 Huang Dafang(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences, Beijing 100081, China);Research and Prospects for Genetically Engineered Agricultural Microorganisms[J];Journal of Agricultural Biotechnology;2003-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 HE Dong-dong, ZHANG Kun-sheng (Tianjin Key Laboratory of Food Biotechnology, Department of Food Engineering, Tianjin University of Commerce, Tianjin 300134);Candida utlis growth optimization[J];Food Science and Technology;2010-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 JIANG Shou-qun 1, WU Tian-xing 2(1.Laboratory Animal Centre of Zhejiang Province,Hangzhou 310013 ,China; 2.Zhejiang University,Hangzhou 310029 ,China);ADVANCES ON PHYTASE RESEARCH:A REVIEW[J];ACTA ZOONUTRIMENTA SINICA;1999-03
2 YAO Bin 1 FAN Yun liu 2 ( 1Feed Research Institute, 2Biotechnology Research Center,Chinese Academy of Agricultural Sciences,Beijing 100081);Molecular Biology and Gene Engineering of Phytase[J];CHINESE JOURNAL OF BIOTECHNOLOGY;2000-01
3 Huang Zunxi (Department of life Science, Yunnan Normal University, Kunming,650092) Zhang Kechang (Biology Engineering College, Wuxi Light Industry University, Wuxi, 214036);Survey on Groundwork and Application Research of Phytase[J];FOOD AND FERMENTATION INDUSTRIES;1999-02
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