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《Journal of Chongqing University(Natural Science Edition)》 2004-11
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Analysis on the HepialusGonggaensis' IntestinalBacterialFlora

ZHUO Feng-ping~(1),CHEN Shi-jiang~(2),YIN You-ping~(1),WANG Zhong-kang~(1),XIA Yu-xian~(1)f Chongqing,of Education,Chongqing University, Chongqing 400030, China;2.Biological Medicine and Cultivation Department,Research Institute of Chinese Herb Medicine, Chongqing, Chongqing 400065, China)  
The larvae of Hepialus gonggaensis are predominant host of Cordyceps sinensis, which can produce one kind of famous and expensive herb medicine in China and Asia area. It is important to determinate the intestinal microbial flora of larva for studying the nutritional physiology and improving the artificial breeding and increasing the larvae livability, which is in turn to benefit the artificial or half artificial cultivation of Cordyceps sinensis. In the experiment, intestinal microbial flora of H. gonggaensislarvae, collected from Kangding,Sichuan Province, have been isolated and identified. The results show that the intestinal microbial flora of H. gonggaensisbelongs to low-temperature bacteria. The bacteriagrew very slowly and the typical bacterial colony appeared on cultural plate only after 2 daysincubatedunder the most profitable temperature,15 ℃。Twelve different isolates of bacteria are isolated from the larve intestine by 5 sorts of cultural media. It shows that the predominant bacterium is Staphylococcus, whose viable count is 2.05×10~(9) mL~(-1), and the detection rate is 100%. It indicates that they should be the normal flora in the larva's intestine. The other strains can be isolated occutionath, they are supposed to be the passer microbes. The biochemical identity shows that the identifying values are relative low for most bacteria, which are consistent with the larvae's abnormal living environment.
【Fund】: 国家自然科学基金资助项目(302440067);; 重庆大学引进人才基金资助项目(200108)
【CateGory Index】: Q939.11
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