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《Fujian Journal of Animal Husbandry and Veterinary》 2007-S1
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Research and application on rapid detection kit of salmonella by universal PCR

Kong Fande1 Chen Qiong2 Xu Shufei1 Peng Haibing3 (1 Xiamen entry-exit inspection and quarantine bureau,Xiamen 361012; 2 Xiamen agricultural product quality and safety testing center,Xiamen 361009; 3 College of Animal science,Fujian agriculture and forestry university,Fuzhou 350002)  
This study evaluated universal PCR to detect Salmonella species according to fimY gene.The specific primers to the fimY gene were used to amplify standard bacteria of A-F salmonella,60 salmonella separate strains and 11 non-salmonella species by PCR. Experiment results indicated that only Salmonella species generated the specific 526 bp DNA fragments on the 2.0% agarose gel and non- salmonella species controls gave negative results,demonstrating that the primers should be specific for Salmonella genus. The PCR was able to detect as little as 93 cfu Salmonella and pre-cultured(BP) or cultured(MM) bacteria liquid of the sample obtaining 3 cfu Salmonella.This indicates established PCR method is high sensitive and strongly specific.The nucleic acid is puried by five methods,including adding bacteria,hot cleavage,freezing-thaw again and again,CTAB and column reagent kit,the CTAB method IS best,but testing procedure is multifarious.Adding bacteria and hot cleavage can procure the same result as column reagent kit and have many merits including simple,rapid,economical and is worthy to generalization and application.
【Fund】: 厦门市科技计划项目(3502Z20042021)资助
【CateGory Index】: S854.43
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【References】
Chinese Journal Full-text Database 4 Hits
1 WANG Yu,WANG Zhen-qiang,ZHANG Wei* (College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China);Loop-mediated Isothermal Amplification Assay for the Detection of Salmonella in Meat and Meat Products[J];Food Science;2010-16
2 YUAN Wei,TANG Shan-hu*,CEN Lu-jia,LI Xue,CHEN Nuo,LUO Wei (College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China);Detection of Salmonella typhimurium in Duck-slaughtering Chain by PCR[J];Food Science;2010-22
3 ZHANG Wei-fan;Institute of Quanzhou Products Quality Inspection;;Research Progress on the Rapid Detection of Salmonella[J];Taiwan Agricultural Research;2013-04
4 LI Xiao-ling,LIU Bin,DAN Xian-long,WANG Da-peng,ZHOU Min,SHI Xian-ming(School of Agriculture and Biology,Shanghai Jiaotong University/MOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center,Shanghai 200240);Development and Application of an IAC-PCR Kit for the Rapid Detection of Salmonella spp.[J];Scientia Agricultura Sinica;2011-16
China Proceedings of conference Full-text Database 1 Hits
1 Xia Mengyun Yang FaLong Zhang Huanrong Yu Hua(College of Life Science and Technology,Southwest University for Nationalities,610041);Advances in Methods for Rapid Detection of Salmonella[A];[C];2008
【Citations】
Chinese Journal Full-text Database 2 Hits
1 YING Cheng-yu et al (Station of Veterinary Health and Quarantine station in Huzhu.Huzhu 810500);Using latex Agglutination Text Detecting Salmonella in Commercial Feed[J];Chinese Qinghai Journal of Animal and Veterinary Sciences;2006-02
2 LeiFeng,Han Zhihui,Zhang Hongjian(Faculty of Agriculture and Animal Sciences,Qinghai University,Xining 810003);Checking Salmonella in Animal Feed by Dot-ELISA[J];China Dairy Cattle;2005-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Qing-ning1,HU Ming2,ZHU Rong-sheng2,REN Xiang-quan3,WU Ying2,WANG Huai-zhong2,LIU Yu-qing2,WANG Shu-bai1(1.Qingdao Agricultural University,Qingdao 266109,China;2.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Livestock Technology Company of Dilisi Group,Zhucheng 262216,China);Microbial Properties and Application of Superior Bacteria in Deep-litter Ecosystem for Piggery[J];Shandong Agricultural Sciences;2009-04
2 JIANG Jian-jun et al(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003);Isolation and Identification of Rabbits Shigella Dysenteriae in a Large Scale Warren[J];Journal of Anhui Agricultural Sciences;2005-09
3 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
4 LIU Su-xia et al(Henan Normal University,Xinxiang,Henan 453007);Study on Optimization for RAPD Reaction System and Genetic Relationship in Punica granatum[J];Journal of Anhui Agricultural Sciences;2007-36
5 ZHANG Lu (Shanxi Agricultural University, Taigu, Shanxi 030801);Discussion on the Detection Methods of Salmonella in Food[J];Journal of Anhui Agricultural Sciences;2008-02
6 CHANG Xin-yao et al(Department of Animal Science,Henan Institute of Technology,Xinxiang,Henan 453003);Study on the Biochemistry Characteristic and Pathogenicity of E.coli and Its Drug Sensitivity Test[J];Journal of Anhui Agricultural Sciences;2008-11
7 JIA Ren-yong et al(College of Animal Science and Technology,Sichuan Agricultural University,Ya’an,Sichuan 625014);Effects of Prinsepia utilis Rogle Polysaccharide on the Serum Antibodies and Blood Biochemical Indices in Chicken[J];Journal of Anhui Agricultural Sciences;2008-17
8 CHANG Hong-tao et al(College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou,Henan 450002);Research on Some Microbial Properties and Drug Resistance of Pathogenic Streptococcus in Henan Province[J];Journal of Anhui Agricultural Sciences;2008-22
9 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance of Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-23
10 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance for Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-24
China Proceedings of conference Full-text Database 10 Hits
1 CHEN Zhen1,Wang Chun-he1,Zhen Liang-ming2,Zhu Chun-hua1,Lin Shu1,LIU Bing-qiong1,LIN Yu1,HUANG Yu1,2(1.Institute of Animal Husbandry and Veterinary,Fujian Academy of Agricultural Sciences,Fujian Fuzhou 350013,China;2.Fuzhou Da Bei Nong Biotech Co.,Ltd Fuzhou 350014,China);MOLECULAR CHARACTERISTICS OF AN H9N2 AVIAN INFLUENZA VIRUS CK/AH/1/10 ISOLATED IN ANHUI PROVINCE[A];[C];2012
2 MAO Li,LI Wen-liang,YAN Shao-hua,ZHONG Sheng,GUO Jing-yao,ZHANG Wen-wen,JIANG Jie-yuan(1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences;Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture;National Center for Engineering Research of Veterinary Bio-products;Nanjing 210014;China 2.Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences 3.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences);Detection of bovine viral diarrhea virus from goat stillbirth[A];[C];2012
3 LIU Yang2,BU Ri-e1,WU Jin-hua1,XUE Xiao-yang2,GUO Chuang1,XI Lin gao wa1,LIU Yan1(1.College of Life Science,Inner Mongolia University for Nationalities,Tongliao 028043,Inner Mongolia,China;2.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028043,Inner Mongolia,China);PCR Determination and Drug Resistance Analysis of E.coli from Diary milk In Inner Mongolia[A];[C];2012
4 XU Hui~(1,2),WEI Li~1,TONG Rui-ping~1,GONG Yu-mei~1, WANG Hong-jun~1,SUN Hui-ling~1,ZHANG Pei-jun~1.(1.Institute for Animal Husbandry and Veterinary Science, Beijing Academy of Agriculture and Forestry Science,Beijing 100097;2.College of Life Science,Capital Normal University,Beijing 100037,China);Development of PCR kit for Mannheimia haemolytica[A];[C];2009
5 KONG Fande1,WANG Rong2,CHEN Qiong3 ,WU De’feng2,XU Shufei1 (1. Xiamen Entry-exit Inspection and Quarantine Bureau, Xiamen 361012, China; 2. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 351006, China;3. Marine Fisheries Department of Fisheries College Jimei Universiy, Xiamen 361021, China);Establishment and primary application of Multiplex Polymerase ChainReaction for Detection of Classical Swine Fever and Porcine Reproductive and Respiratory Syndrome Virus[A];[C];2008
6 MA Xiao-dong,CHEN Shan-shan,LI Chong-jiu (Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100094,China);Study on Deactivation of Tobacco Mosaic Virus Coat Protein by Propionyl Shikonin[A];[C];2008
7 Zhao Heng-zhang Liu Bao-guo Zhao Kun Li Guo-wang Du Wei-guo (Henan Institute of Science and Technology,Xinxiang 453003);Antibiosis Effect of Chinese Medicinal Herbs on Haemophilus Patasuis such as Rhizom Coptidis,Flos Lonicetae Japonicas,Etc[A];[C];2008
8 Chang Hong-tao Wang Chuan-Qing Chen Lu Yao Xue-Jing (The College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou,450002);Pathogenic Streptococcus Suis in Henan Province Some Microbial Properties and Resistance Research[A];[C];2008
9 Liang Hong-de Wang Rui-jian Yang Yu-rong (Henan Agriculture University Animal Pathology Group,Zhengzhou 450002);Pathology Observation of Large Intestine of Rabbits Infected with Rabbit Hemorrhagic Disease Virus[A];[C];2008
10 ;低温炮制工艺对紫河车水溶性蛋白组成及其纤溶活性的影响[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Lu (Shanxi Agricultural University, Taigu, Shanxi 030801);Discussion on the Detection Methods of Salmonella in Food[J];Journal of Anhui Agricultural Sciences;2008-02
2 YE Gang et al(College of Animal Medical,Sichuan Agricultural University,Yaan,Sichuan 625014);Study on Effect of Eliminating the Main Pathogenic Bacteria of Cow Mastitis by Ruyankang Injection on Drug Resistance of β-lactam Antibiotics[J];Journal of Anhui Agricultural Sciences;2008-26
3 CAO Guo-qing et al(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi 030801);Genomic DNA Isolation by Phenol/Chloroform Extracting Method from Sheep Blood Clot[J];Journal of Anhui Agricultural Sciences;2009-34
4 HAN Zhi-hui et al(Department of Animal Medicine.Qinghai University,Xining,Qinghai 810016);Detection Salmonella in Forage by Dot-ELISA[J];Journal of Anhui Agricultural Sciences;2011-26
5 XIA Chun* 1; LIU Jin 1, HUANG Jie 2 (1. Department of Microbiology and Infection, College of Veterinary Medicine, China Agricultural University. Beijing 100094, China; 2. Yellow Sea Fisheries Research Institute, Shandong, Qingdao 266071, China);Detection of Hypodermal and Hematopoietic Necrosis Baculovirus of Penaeid Shrimp by One-tube Multiplex PCR Method[J];CHINESE JOURNAL OF VIROLOGY;2000-03
6 LI Qi-ming~1,MA Xue-jun~1,2,ZHOU Rui~1,PENG Fu-wang~1,GAO Han-chun~2,KUANG Zhi-zhou~1,HOU Yun-de~1,2(1.State Key Laboratory for Molecular Virology and Genetic Engineering,Beijing 100052,China;2.Beijing Jindike Biotech Institute,Beijing 100176,China);Detection of HCV Gene by Reverse Transcription Loop-mediated Isothermal Amplification Method[J];Chinese Journal of Virology;2006-05
7 SHI Si YING Yu-Min SHA Jie YANG Shu-Qing(Lucheng Zone Sanitation and Epidemic Prevention Station Zhejiang Wenzhou 325000,China);Investigation of Bacteria Contamination in the Pet Food[J];Chinese Journal of Zoology;2001-06
8 WANG Shao~1,LIU Gui-hua~2,QIAO Feng~1,SHI Jie-ping~1,FANG Li~3,LI Bo~ 1* (1. Department of Epidemiology,School of Public Health,Jilin University,Changchun 130021,China; 2. Center for Disease Prevention and Control of Jilin Province,Changchun 130012,China;3. Health and Epidemic Prevention Station,Jilin Petroleum Combine Limited-Liability Corporation ,Songyuan 138000,China);Establishment of PCR method for detection of Staphylococcus aureus[J];Journal of Jilin University(Medicine Edition);2006-05
9 ZHANG Li.Research Institute for Prevention and Treatment on Dermatology,Guangzhou 510060,China;Quality Control of Laboratory on Polymerase Chain Reaction in Hospital[J];Journal of Bethune Military Medical College;2005-03
10 WANG Juan,WANG Yu-dong,ZHENG Zeng-ren,ZHAO Si-jun,HUANG Xiu-mei (China Animal Health and Epidemiology Center,Qingdao 266032);Study on Susceptibility for Avian E.coli and Salmonella[J];Poultry Science;2009-06
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Chen Qing-hua;Pingtan Quality and Metrology Inspection Institute;;Research on Application of PCR and Improved PCR Technology in Food Safety Detection[J];Fujian Analysis & Testing;2014-02
2 QIU Suo-ping;LIN Zhi-xiong;YOU Yong-lai;MA Bao-hua;ZHAO Qiong;Conghua Entry-exit Inspection and Quarantine Bureau;Guangdong Entry-exit Inspection and Quarantine Bureau;;Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys[J];China Animal Husbandry & Veterinary Medicine;2014-01
3 Cheng Xiao;Wang Yongxin;An Hong;Liu Juanjuan;Zhang Bo;Jia Zhen;Cheng Jian;China National Center for Quality Supervision and Test of Agricultural-avocation Processed Food ( Anhui);Anhui Inspection and Research Institute for Food and Drug;;Detection of Staphylococcus aureus in Dairy Food by Loop-mediated Isothermal Amplification[J];动物与饲料科学(英文版);2014-03
4 WANG Sheng-qi;LI Ai-hua;GUO Qi;ZHANG Lei;LI Jing;LIU Yan-jun;LI Li;Henan International Travel Healthcare Center;;Evaluation of two methods of genetic analysis for detection Salmonella in port sanitary inspection[J];Chinese Journal of Frontier Health and Quarantine;2015-03
5 LI Feng-mei;SHI Kai-chuang;XU Xin-ting;HU Jie;ZOU Lian-bin;ZHONG Cheng;College of Animal Science and Technology,Guangxi University;Guangxi Center for Animal Disease Control and Prevention;;Establishment and Application of a Multiplex PCR Assay for Detection of Pathogenic Salmonellafrom Chickens[J];Progress in Veterinary Medicine;2015-08
6 ZHU Ru-gang1,SONG Li-feng2(1.Shenyang Key Laboratory of Food Biology Processing and Quality Control Technology,Engineering Technology Research Center for Food Biology Processing of Liaoning Province,College of Light Industry,Liaoning University,Shenyang 110036,China; 2.Liaoning Economic Management Cadre Institute,Shenyang 110122,China);Detection of Viable Salmonella Cells in Meat and Meat Products by PCR[J];Food Science;2012-16
7 Li Xue-ling,Chen Yong,Zhang Jian Shanxi Provincial Institute of Product Quality Supervision and Inspection(Xi’an 710054);Application of Internal Amplification Control in the PCR Detection Method for Foodborne Pathogens[J];The Food Industry;2013-04
8 TIAN Wen-Li*,QI Ji,YANG Xu,LI Xiao-Qiang Tasly & Jenner(Tianjin) Co.,Ltd,Tianjin 300410,China;Establishment of Rapid Detection for Salmonella in Influenza Virus Subunit Vaccine Intermediates[J];Letters in Biotechnology;2013-03
9 SHAO B i-ying;CHEN Wen-bing;ZENG Ying;CHEN Bin;ZHENG Jing;MIAO Ting-yu;PENG Juan;Fujian Entry-Exit Inspection and Quarantine Bureau;College of Food Science,Fujian Agriculture and Forestry University;;Detection of Genetically Modified Soybean Line A2704-12 in Soybean and Its Products by LAMP Method[J];Food Science;2013-24
10 FANG Ting-Zi;SHI Chun-Lei;MOST-USDA Joint Research Center for Food Safety,School of Agriculture and Biology,Shanghai Jiao Tong University;;Application of molecular techniques in detection of foodborne pathogenic microorganisms[J];Journal of Food Safety & Quality;2014-07
China Proceedings of conference Full-text Database 1 Hits
1 QI Ji, TIAN Wen-li, GAO Yan-yan, Yang Xu (Tasly & Jenner(Tianjin) Co., Ltd, Tianjin 300410, China);A Rapid PCR Detection Method for Salmonella in Influenza Virus Subunit Vaccine Intermediates[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 HUANG Jin lin, JIAO Xin an, WEN Qi yi, PAN Zhi ming, ZHANG Xiao rong, ZHANG Ru kuan, LIU Xiu fan ( Lab of Ani Infect Dis, MOA, Yangzhou Univ, Yangzhou 225009, China);APPLICATION OF POLYMERASE CHAIN REACTION FOR RAPID DETECTION OF SALMONELLA[J];Journal of Yandzhou University;2002-03
2 Liu Yongde, Wang Yi, Xu Jun, Shi Caipei, Chen Zhilong ( Department of Animal Science, Shanghai Agricultural College );STUDY ON COMPARISON OF THREE RAPID DIAGOSIC METHODS FOR RABBIT HEMORRHAGIC DISEASE[J];Chinese Journal of Animal and Veterinary Sciences;1992-04
3 Chen Xin, Lin Qian, Yang Ying (College of Life Life Science, Shanghai University, Shanghai 200436);Isolation and Idontification of Salmonella spp. form Imported Feedstuff of Animal Origin[J];Chinese Journal of Animal Quaranitine;2002-01
4 Xie Yijun,et al(Fujian Health and Anti-epidemic Station,Fuzhou 350001);First Discovering of Salmonella Ⅲ_b with K Antigen (48:a:Z55) in China[J];CHINESE JOURNAL OF ZOONOSES;1994-04
5 Wen Qiyi ;Jiao Xi′nan; Liu Xiufan ;Zhang Rukuan; Yang Jing(Agricultural College of Yangzhou University, Yangzhou,Jiangsu 225009);A Direct ELISA for the Detection of Salmonella spp.inDifferent Specimens[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1995-02
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