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《Fujian Journal of Animal Husbandry and Veterinary Medicine》 2008-01
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Detection of Proteus mirabilis in animal-derived food by Polymerase Chain Reaction

Luo Zhaofei1 Zhang Weiyu1 Yang Desheng2(1. The faculty of animal science and technology,Guangxi university,Nanning 530022;2. Center for prevent and control the animal diseases in Fujian province,Fuzhou 350003)  
To establish Polymerase Chain Reaction (PCR) method for detection Proteus mirabilis,so as to provide a reference method for sanitary inspection.On the basis of the conserved section of the tellurite resistance gene sequence of Proteus mirabilis,a sets of specific primers was designed,a PCR method developed to identify Proteus mirabilis. The PCR method established in this article are highly specific,and it can detect Proteus mirabilis 1.2×102 cfu/100g dried meat in sliced form by amplifying a 248 bp DNA fragment.The PCR assay was rapid,reliable,specific and sensitive,and the method can be used in the detection of Proteus mirabilis in animal-derived food.
【CateGory Index】: R155.3
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