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《Fujian Journal of Animal Husbandry and Veterinary Medicine》 2008-S1
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Studies on dot immunogold filtration assay for detection of antibody of Hog cholera virus and porcine reproductive and respiratory syndrome simultaneously

Wang Rong1,2 Kong Fande1* Chen Qiong3 Wu Defeng2 Xu Shufei1 (1. Xiamen Entry-exit Inspection and Quarantine Bureau,Xiamen 361012,China; 2. College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China; 3. Xiamen Agricultural Product Quality and Safety Testing Center,Xiamen 361009,China)  
On the basis of single dot immunogold filtration assay (sDIGFA) for detection of antibody of HC or PRRS,double DIGFA which could detect HC and PRRS antigen simultaneously was established. The purified antigens of HCV and PRRS were coated on nitrocellulose (NC) membrane,and staphylococcal protein A (SPA) labelled with colloidal gold was used for reaction reagent. As swine sera contain both HC and PRRS antibodies,the color of the positive dots (red dots)were so bright that the results could be easily and quickly determined. Furthermore,this method had a better specificity without cross-reaction with positive sera to PPV,FMD and PRV.
【Fund】: 厦门出入境检验检疫局科技计划项目(XK09-2003)
【CateGory Index】: S858.28
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