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《Fujian Analysis & Testing》 2006-04
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Separation of Luohanguo Glucoside by HPLC

Ma Shaomei ( Department of Chemistry and Ecology Engineering, Guangxi University for Nationalities, Nanning, 530006, China)  
Objective:To establish a method for quality evaluation of mogroside. Method: The strengthened ultrasonic extraction technology was used for the sample pretreatment. Active component peak was located by the reference compound mogrosideV. Mogroside aqueous extracts were separated by HPLC. The effects of isocratic elution and gradient elution for six moving phase systems, i.e. water-methanol, acetic acid-methanol, phosphoric acid-methanoic acid, water- methyl cyanide as well as phosphoric acid- methyl cyanide and trifluoroacetic acid- methyl cyanide were compared. Result: The better separated conditions are using trifluoroacetic acid- methyl cyanide as moving phases of the gradient elution under the detected wavelength of 205 nm, flow rate of 0.8mL/min and column temperature of 25℃ using a column of Sinochrom ODS-BP. Conclusion: The chromatogram of mogroside aqueous extracts with better resolution and repeatability can be obtained by HPLC.
【Fund】: 广西教育厅自筹资金(2003)
【CateGory Index】: R284
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