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《Fujian Analysis & Testing》 2012-01
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Rapid hydrolysis Method for the Determination of Tryptophan in Feather and its Degradation Products

Chen Xiang-qun1,Lin Xiang-xin2(1 Fujian Chemical School,Xiamen,Fujian 361004,China; 2 Central Laboratory of Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350001,China)  
The pretreatment method of increasing the hydrolysis temperature and reducing the hydrolysis time,determinating the tryptophan contents in the feather and its degradation products by automatic amino acid analyzer.Analysis results show that the tryptophan contents determined by 4mol/L LiOH and 5h hydrolysis at 145℃ was the same as that from conventional method.The new method obtained a recovery rate of 91% with the highest repeatable CV 2.24%.It can not only shorten the analysis time,and does not affect the accuracy of the results.
【CateGory Index】: S816
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