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《Fujian Analysis & Testing》 2012-03
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Determination of Two Diarrhetic Shellfish Poisons Residues in Mytilus edulis Linnaeus by High Performance Liquid Chromatography Tandem Mass Spectrometry

Zhang Hai-qi1,He Xin2,Zheng Chong-ying1(1.Zhejiang Fisheries Quality Testing Centre,Hangzhou,Zhejian,310012,China; 2.College of Food Science and Biotechnology Engineering,Zhejiang Gongshang University,Hangzhou Zhejian,310035,China)  
Establish the detection method of okadaic acid(OA) and dinophysistoxins(DTX-1) in Mytilus edulis using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Shellfish sample was extracted by 80% methyl.Shellfish extract solution was clean-up by Sep-pak silica column.A triple-quadrupole tandem mass spectrometer was used as a detector for HPLC to determine OA.As for the MS/MS,the multi-reactions monitoring(MRM) scan type and the negative ion electrospray ionization(-ESI) mode were applied.The precursor ion→product ion(m/z 803.5→m/z 255.1,m/z 803.5→m/z 563.1) was selected as OA quantitate detection ion pair.The precursor ion→product ion(m/z 817.4→m/z 255.1,m/z 817.4→m/z 113.1) was selected as DTX-1 quantitate detection ion pair.The mobile phase of HPLC was the methyl cyanide: 1% methanoic acid-water(vol/vol: 70:30) and the chromatographic column was Zorbax XDB-C18(2.1mm×150mm×5μm).The standard OA and DTX-1,the shellfish being added with OA and DTX-1,the shellfish sample were determine by the HPLC-MS/MS.The standard curve for OA showed good linearity over the concentration range of 5~640 ng/ml,the equation of linear regression was Y =207X-241(Q1/Q3:m/z 803.5→255.1),r was 0.9998,The recovery of sample being added with OA was 79.5%~88.6% and the RSD was 8.43%~10.4%.The standard curve for DTX-1 showed good linearity over the concentration range of 0 ~ 200 ng/ml,the equation of linear regression was Y =141X +3.59×103(Q1 /Q3: m / z817.4→255.1),r was 0.9997,The recovery of sample being added with DTX-1 was83.8%~91.2% and the RSD was 4.22%~6.54%.This HPLC-MS/MS is highly sensitive,fast,and very accurate.So it can be used for detecting the remain of OA and its natural derivative DTX-1 in shellfish.Among the 45 samples from the origin and market,4 samples were detected DSP,the ratio was 8.9%.
【Fund】: 浙江省重点科研社会发展项目(№2007C23081)
【CateGory Index】: R155;O657.63
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 MU Qing-Lin* 1,FANG Jie1,WAN Han-Xing2,WANG Xiao-Hua1,CAO Liu-Yan1,ZHANG Qing-Hong1 1(Zhejiang Provincial Zhoushan Marine Ecological Environmental Monitoring Station,Zhoushan 31600) 2(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100);Determination of Diarrhetic Shellfish Poisoning in Shellfishes by Liquid Chromatography with Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2011-01
2 Wang Juying; Guan Daoming;Han Gengchen(National Marine Environmental Monitoring Center,Dalian,116023);Analytical Methods for Diarrhetic Shellfish Poisoning[J];MARINE ENVIRONMENTAL SCIENCE;1996-04
3 ;Review on research of diarrhetic shellfish poisoning[J];Marine Sciences;2005-05
4 ZHAO Qian-cheng1,2, LAI Peng2,3, QIN Cheng2,3, WANG Chong4, WU Bin3, LIU Di2, CHANG Ya-qing1* (1.Key and Open Laboratory of Mari-culture and Biotechnology, Dalian Fisheries University, Dalian 116023; 2. Key and Open Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, College of Food Engineering, Dalian Fisheries University, Dalian 116023; 3. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001; 4. College of Food Science, Shenyang Agricultural University, Shenyang 110161);Diarrihetic shellfish poison detecting in scallop by ELISA and mouse biological method[J];Food Science and Technology;2009-11
5 LI Xiao-zheng,HUANG Yu-liu,WU Xiang-qing,PANG Yan-fei,WEI Xin-xian,HUANG Guo-qiu (Guangxi Fishery Inst.,Nanning 530021);Cause Analysis of Error in Bio-Detection of Diarrhoeal Shellfish Poisons[J];Journal of Microbiology;2009-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Qian et al(Department of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240);A Review for Hazards and Determination Methods of Amnesic Shellfish Poison——Domoic Acid[J];Journal of Anhui Agricultural Sciences;2011-26
2 ZHOU Xiangdong1,CHEN Huibin2,ZHAN Shuyue1,WANG Xiaoping1 OU Huichao3,LUO Zhaofeng3 1.State Key Lab.of Modern Optical Instrumentation,Zhejiang University,Hangzhou 310027,China;2.Depatrmant of Information science and engineering,Jimei University,Xiamen Fujian 361021,China;3.Hefei National Lab.for Physical Sciences at Microscale,University of Science and Technology of China,Hefei 230027,China;Research of a SPR Immunoassay Method for Okadaic Acid Contamination of Red Tide Toxins[J];Chinese Journal of Sensors and Actuators;2011-12
3 DU Wei,LU Dou-ding(Second Institute of Oceanography,SOA,Key Laboratory of Marine Ecosystem and Biogeochemistry,SOA,Hangzhou 310012 China);Harmful effects and detection of toxic algae and their algal toxins[J];Journal of Marine Sciences;2008-02
4 ZHANG Shaojun~1, DING Yongsheng~1, LI Dazhi~2, MA Xu~1,SUN Li~2(1.Environmental Science & Eng.College, Dalian Maritime Univ., Dalian 116026, China;2.Dalian Customs Lab., Dalian 116021, China);Advances on analysis and determination of shellfish toxins[J];Journal of Dalian Maritime University;2003-04
5 LIU Ren-yan1,2,3,LIANG Yu-bo2,3,ZHANG Fang2,XU Dao-yan2,3,MA De-yi1,2,3(1.College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China;2.National Marine Environmental Monitoring Center,Dalian 116023,China;3.SOA Key Lab of Coastal Environment and Ecosystem Research,Dalian 116023,China);Enzyme-linked immunosorbent assay method for detection of diarrhetic shellfish poison in shellfish along the coast of china[J];Journal of Dalian Maritime University;2008-02
6 DING Jun (Key Laboratory of Maricultural Ecology, Ministry of Agriculture, PRC,Dalian Fisheries Univ , DaLian 116023,China);A review: paralytic shellfish poisoning and diarrhetic shellfish poisoning in red tide poison[J];Journal of Dalian Fisheries University;2001-03
7 Li Shubing Li huizen Xuxiping;Primary study on present status of shellfish toxicity and its source[J];Journal of Fujian Fisheries;2000-01
8 FENG Zhen-Zhou1,2,YU Ren-Cheng1,ZHOU Ming-Jiang1 1(Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071,China) 2(Graduate School,Chinese Academy of Sciences,Beijing 100039,China);Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum LimaDodge[J];Chinese Journal of Analytical Chemistry;2012-04
9 Xu Couyou,Huang Qixiang,Hu Yanhua,Chen Zhangxu*(Department of Environment and Life Sciences,Putian University,Putian 351100,China);Research of Toxicated Mechanisms of Red Tide Phycotoxins and Its Symptom[J];Guangdong Chemical Industry;2010-04
10 LI Zhao-Yong1,CHEN Jun-Hui1,LI Xin1,ZHANG Ru-Tan1, CHEN Chen1,SHI Qian1,WANG Shuai1,ZHENG Li1,WANG Xiao-Ru1,2 1(Research Center for Marine Ecology,The First Institute of Oceanography, State Oceanic Administration,Qingdao 266061,China) 2(College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China);Rapid Screening,Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database[J];Chinese Journal of Analytical Chemistry;2013-07
China Proceedings of conference Full-text Database 3 Hits
1 ;广州市水产市场“带子”中贝类毒素的检测[A];[C];2010
2 Yang Li,Yang Wei-dong,Liu Jie-sheng,Jiang Tao College of Life Science and Technology,Jinan University,Guangzhou 510632,China;Analysis of paralytic shellfish poisoning and diarrhetic shellfish poisoning of bivalves in seafood market of Guangzhou[A];[C];2006
3 Lin Chao,Lu Shi-Ying~*,Wang Dong-Xu,Ren Hong-Lin,Zhou Yu,Liu Zeng-Shan~* Key Laboratory for Zoonosis research,Ministry of Education,Institute of Zoonosis,Jilin University,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,P.R.China.;Detection Okadaic Acid from seafood market along the coast of China[A];[C];2012
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 DING Jun (Key Laboratory of Maricultural Ecology, Ministry of Agriculture, PRC,Dalian Fisheries Univ , DaLian 116023,China);A review: paralytic shellfish poisoning and diarrhetic shellfish poisoning in red tide poison[J];Journal of Dalian Fisheries University;2001-03
2 Li Aifeng~ 1,2 , Yu Rencheng~ *1 , Li Jun~1, Tang Xianghai~ 1,2 , Wang Yunfeng~1, Yan Tian~1~1, Zhou Mingjiang~1 ~1(Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology,Chinese Academy of Sciences, Qingdao 266071) ~2(Graduate School, Chinese Academy of Sciences, Beijing 100039);Protein Phosphatase Inhibition Assay for Detection of Diarrhetic Shellfish Poison in Oyster[J];Chinese Journal of Analytical Chemistry;2006-03
3 JIANG Tao1,2,3,JIANG Tian-Jiu3,41(Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071)2(The Graduate School,Chinese Academy of Sciences,Beijing 100039)3(Department of Environmental Engineering,Jinan University,Guangzhou 510632)4(College of Life Science and Technology,South China Normal University,Guangzhou 510631);Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish[J];Chinese Journal of Analytical Chemistry;2008-11
4 LIU Ren-yan1,LIANG Yu-bo1,CHEN Yuan2,XU Dao-yan1,CHEN Bing-jun1,LIU Yong-jian1(1.National Marine Environmental Monitoring Center,Dalian 116023;2.Zodolabs Biotech CO.,LTD.,Nanchang 330029);Development of Gold Immunochromatography Assay for the Detection of Okadaic Acid[J];Journal of Analytical Science;2010-01
5 LIU Ren-yan1,2,LIANG Yu-bo*2,CHEN Bing-jun2,XU Dao-yan2 and LIANG Bin2(1.Environmental Science and Engineering College,Dalian Maritime University,Dalian 116026;2.National Marine Environmental Monitoring Center,Dalian 116023);Primal study on rapid detection of okadaic acid by immune thin layer chromatography with colloidal gold[J];Chinese Journal of Analysis Laboratory;2008-07
6 ZOU Ying lin 1,2 , ZHU Ming yuan 1,2 , HALL Sherwood 3 (1.First Institute of Oceanography, SOA, Qingdao 266061,China) (2.Key laboratory of Marine Science and Numerical Modeling, SOA, Qingdao 266061,China) (3.Seafood office, Food and Drug;Production and Characteristics of Paralytic Shellfish Toxins in Two Species of Alexandrium[J];Journal of Oceanograpgy of Huanghai &bohai Seas;2001-03
7 Chen Zeling, Fu Yunna and Gong Ning National Marine Environmental Monitoring Center. Dalian 116023;Analysis of Diarrhetic Shellfish Poisoning Toxins by High Performance Liquid Chromatography[J];Marine Science Bulleten;2000-01
8 LIU Ren-yan, FU Yun-na, GUAN Dao-ming (National Marine Environmental Monitoring Center, Dalian116023, China);Determination of diarrhetic shellfish poisoning and amnesic shellfishpoisoning of sea shellfish by HPLC in China Coast[J];Marine Environmental Science;2004-01
9 WU Shi-wei,ZHANG Chun-chao,LU Chu-qian,LIU Jing-qin,LIANG Qian-lin(South China Sea Environment Monitoring Center,SOA,Guangzhou 510300,China);Analysis on diarrhetic shellfish poisoning in shellfish inshore in the South China Sea[J];Marine Environmental Science;2005-04
10 WU Shi-wei1,ZENG Miao2,LU Da-peng1,LUO Yi-dan1,JIANG Tian-jiu3(1.South China Sea Environment Monitoring Center,SOA,Guangzhou 510300,China;2.Research Center of Hydrobiology,Jinan University,Guangzhou 510632,China;3.Research Center of Harmful Algae Blooms & Aquatic Envirionment Science,Jinan University,Guangzhou 510632,China);Analysis on diarrhetic shellfish poisoning of shellfish from coastal area of the Guangdong Province in spring 2005[J];Marine Environmental Science;2008-02
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