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《Environmental Science and Technology》 2001-04
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Optimistical synthetic culture condition of five metal-ion on lignin biodegradation ability of alkaliphilic ligninolytic bacteria using orthogonal experimental design

LUO Yu xuan 1, ZHANG Jia yao 1* , XU Wei ya 2, LING Jie 2 (1.Department of Environmental Science ; 2.Center of Analysis Measurement, Wuhan University, Wuhan 430072)  
The effects of metal ion Mn 2+ ?Cu 2+ ?Fe 2+ ?Zn 2+ and Ca 2+ on lignin biodegradation ability of alkaliphilic ligninolytic bacteria strain 6 in alkaline liquid medium (pH≈10.5) with compounded carbons are optimized using orthogonal experiments. The result show the five metal-ions promote the activities of Laccase and MnP and the degradation rate of wheat straw lignin. The synthetic result is analysised. Mn 2+ and Cu 2+ are the key factors. The optimal synthetic condition can take MnSO 4·H 2O 0.7g/L?CuSO 4·5H 2O 1mg/L?FeSO 4·7H 2O 5mg/L?ZnSO 4·7H 2O 30mg/L and CaCl 2·2H 2O 50mg/L.
【Fund】: 国家自然科学基金资助项目 (2 96 770 12 )
【CateGory Index】: X172
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