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《Environmental Science and Technology》 2005-03
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Inhibition Effect of Benzidine on Alkaline Phosphatase and Its Kinetics Study

LIU Bin~(1), ZHOU Pei-jiang~(1), WU Xin-guo~(1), SONG Li-rong~(2) (1. School of Resource and Environment Science, Wuhan University, Wuhan 430072; 2.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072)  
Spectrophotometry was used to study activity of alkaline phosphatase in the presence and absence of benzidine. Relative activity of alkaline phosphatase was calculated according to the absorbance variation. Results showed the activity of alkaline phosphatase was obviously inhibited by benzidine.The inhibition type and inhibition constant of benzidine on alkaline phosphatase were determined by using double reciprocal plot.The kinetics of substrate reaction with different concentrations of substrate p-notrophenylphostrate (PNPP) and inactivator benzidine suggested a competitive complex mechanism for inactivation by benzidine, which is a competitive inhibitor of alkaline phosphatase,with inhibition constant of 1.28×10~(-7)mol/L.
【Fund】: 国家高技术研究发展计划(863)项目(2001AA641030);; 国家自然科学基金项目(20177018)
【CateGory Index】: X132
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