Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Environmental Science & Technology》 2010-06
Add to Favorite Get Latest Update

Preparation of Acetylcholinesterase by Crucian Carp Brain and Its Sensitivity to Several Organophosphorus Pesticides

JIA Yu-ling1,2, PENG Hui-min1, PENG Fang-yi3, CAI Qiang2, HE Miao4, SHI Han-chang4 ( 1.School of Basic Medicine, Chongqing University of Medical Science, Chongqing 400016, China; 2.Yangtze Delta Region Ecoenvironment Institute of Tsinghua University, Jiaxing 314000, China; 3.School of Chemical and Biological Engineering, Chongqing University of Technology, Chongqing 400050, China; 4.Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China)  
Acetylcholinesterase ( AChE) is one kind of crucial enzyme in nerve conduction. For the serine residue at the active site of AChE could be phosphorylated by organophosphorous pesticides ( OPs) , AChE could be used as the target enzyme for OPs nerve positions. Here described the purification and characterization of AChE from carp brain. Firstly, the crude AChE from the crucial carp brain was extracted, and then the enzyme properties including enzyme activity, sensitivity and detection limit were analyzed in the optimized conditions. After purification of crude enzyme by chromatography, the activity of enzyme reached 458.43μmol/min/mg while assay conditions were as 200mmol/L substrate, pH 8.0 and bathing for 30min at 30℃. Enzyme activity could be affected by substrate concentration, pH, temperature, time and dose of enzyme in the descending order. Results also indicated dichlorvos showed the most susceptible to OPs, and then trichlorfon, acephate, tiazophos, poxim and methoate.
【Fund】: 国家高技术研究发展计划(863)项目资助(2007AA06Z419);; 浙江省重点科技计划项目资助(2006C23069);; 浙江省自然基金项目资助(Y507081)
【CateGory Index】: X839.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 YI Mei-qin 1,3, YU Cai-hong2, YANG Ming2, JIANG Hui4,LIN Rong-hua4, GAO Xi-wu1(1 Department of Entomology, China Agricultural University, Beijing 100094, China; 2 College of Chemistry and Environment Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China; 3 Collage of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, China; 4 Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing 100026, China);Tissue distribution of Cholineserases and its sensitivity to carbamate insecticides in Carassius auratus[J];Journal of Safety and Environment;2006-03
2 ZHAO Jian-Zhuang et al(Beijing Agricultural College ,Beijing 102206);Study on Parition and Purification of Acetylcholinesterase[J];Journal of Beijing Agricultural College;2003-04
3 WANG Ye1,2,PENG Yuan2,ZHAO Su-qing3,XU Feng-cai4(1.Sinoasis Pharmaceuticals Inc,Guangzhou,Guangdong,510760,China;2.Guangxi Academy of Sciences,Nanning,Guangxi,530007,China;3.Guangxi Institute of Botany,Guilin,Guangxi,541006,China;4.College of Life Science,South China Agricultural University,Guangzhou,Guangdong,510642,China);The Nature of Acetylcholinesterase Membrane and Its Detection Effects on Organophosphorus Pesticides[J];Journal of Guangxi Academy of Sciences;2007-03
4 LIU Xiao-yu1,OUYANG Xue-sheng1,WU Mou-cheng1,YANG Jian2,XU Ying3(1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China; 2.Key Laboratory of Ecological Environment and Resources of Inland Fisheries/Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China; 3.Key Laboratory of Freshwater Ecology and Biotechnology/Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China);Study on Parition,Purification and Activity of Acetylcholinesterase in Crucian Muscle[J];Hubei Agricultural Sciences;2007-04
5 HE Donghai, MENG Fanping, ZHU Xiaoshan (Department of Environmental Ecology, Ocean University of China, Qingdao 266003, Shandong, China);Advances in the Study on Toxic Effect of Organophosphorus Pesticides on Acetylcholinesterases (AChE) of Marine Animals[J];Marine Science Bulletin;2003-06
6 XIE Xian chuan, LI Shao nan *, ZHU Guo nian, TAN Ya jun (Department of Plant Protection, Zhejiang University, Hangzhou 310029, China);Purification of Brain Acetylcholinerase (AChE)from Topmouth Gudgeon and Comparative Studybetween Crude and Purified AChE[J];Chinese Journal of Pesticide Science;2003-01
7 ZHU Xiao-shan~(1),MENG FAN-ping~(2),HE Dong-hai~(2)(1.College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China;2.College of Marine Life Science,Ocean University of China,Qingdao 266003,China);Purification and Partial Biochemical Characterization of Acetylcholinesterase from Scomberomorus Niphonius(Cuvier)[J];Journal of Qingdao University(Engineering & Technology Edition);2006-02
8 LIU Xiao-yu1,HAO Qiang1,WU Mou-cheng1,YANG Jian2,XU Ying3 (1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;3.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China);Study on the Determination of Brain AChE Activity in Crucian Carp and Its Sensitivity to Organophosphorus Pesticides[J];Food Science;2006-12
9 LIU Shi-wen1,HOU Yun-xiu1,CHEN Jia-rong1,F U Feng-hua2 (1.Affiliated Hospital of Binzhou Medical College,Shandong Binzh ou 256603,China; 2.Department of Immunology,Binzhou Medical College,Shandong Binzhou 2566 03,China);Detection of Free Organophosphates by the Method of Restra ining Purified Acetylcholinesterase Activity[J];Journal of Medern Laboratory Medicine;2005-02
10 TANG Ya-fei~(1,2),WANG Yan-xing~1,CAI He-sen~1,Broder J. Merkel~3 (1.Chinese University of geology,Wuhan 430074,China;2.School of environmental and Civil Engineering, Wuhan Institute of Chemical Technology,Wuhan 430073,China;3.Faculty for Geosciences, Geoengineering and Mining,Freiberg University of Mining and Technology,Freiberg,Germany);Application and pollution of oganophosphorous pesticides[J];Journal of Wuhan Institute of Chemical Technology;2004-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 QIU Chao-kun et al (College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070);Study on the Extraction of Acetylcholinesterase from Chicken Blood[J];Journal of Anhui Agricultural Sciences;2007-19
2 LI Fu-hou et al(Key Laboratory of Marine Biotechnology of Jiangsu Province,School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Research Progress on Microbial Degradation of Organophosphorus Pesticides[J];Journal of Anhui Agricultural Sciences;2009-22
3 LIU Hong-xia,SHI Xue-yan,GAO Xi-wu(Department of Entomology,China Agricultural University,Beijing 100094,China);Establishment of optimization of assaying activity of acetylcholinesterase from Chironomus kiiensis and their sensitivity to organophosphate insecticides[J];Journal of Safety and Environment;2005-05
4 Zhou Shaoyun (ZhangZhou product quality supervision and Inspection institute,Zhangzhou.Fujian 363000,China);Preliminary Study On the Dermination of Pesticide Residuces in Vegetables With Acetylcholinsterase Enzyme (ACHE) Extracted From Tilapia Mossambica[J];Fujian Analysis & Testing;2007-03
5 Li Zhaohua,Wang Xiaohu,Zheng Xiuping,Yin Jibin(Shandong Academy of Environmental Science,Jinan 250013,China);Application of the Modern Biotechnology on the Detectionp of Pesticde and Veterinary Drug Residues[J];Environmental Science and Management;2009-11
6 YE Rong-cun(Fujian Institute of Education,Fuzhou 350025,China);Distribution and Purification of Acetylcholinesterase in Tenebrio Molitor L[J];Journal of Fujian Institute of Education;2010-02
7 WANG Gai-zhen, WEI Fu-xiang, WANG Chang-shi,WANG Xiao-hui, LIANG Gui-yong (College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang Hebei 050018,China;Hebei Qinghe Middle School,Qinghe Hebei 054800,China);Development of enzyme biosensor of organophosphorus pesticides[J];Hebei Journal of Industrial Science & Technaology;2005-04
8 Kang Xiaodan,Meng Fanping,Zhang Aijing.(College of Environmental Science & Engineering,Ocean University of China,Qingdao Shandong 266100);Study on the response conditions of acetylcholinesterase from Scomberomorus niphonius to methyl parathion in seawater[J];Environmental Pollution & Control;2008-11
9 YU Jun,ZHOU Yan (Marine Mornitoring&Forecasting Center of Zhejiang Province,Hangzhou 310012,China);Determination of organophosphorous pesticide residues in marine organisim by gas chromatography[J];Marine Environmental Science;2007-05
10 YU Xiang-yang~1, CHU Rui-wu~1, TIAN Zi-hua~2, LIU Xian-jin~1 (1.Food Safety Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Inspecting and Testing Center for Farm Produce, Jiangsu Agriculture and Forest Office, Nanjing 210036, China);Influences of Enzyme and Extraction Methods on Enzyme-inhibited Fast Assay for Pesticides[J];Jiangsu Journal of Agricultural Sciences;2005-03
China Proceedings of conference Full-text Database 1 Hits
1 WANG Xia,LIU Ming-jun,GAO Jun-e,CHEN Jun-wu,LIU Shun-qin,LI Dun ( College of Natural Resources and Environment,South China Agricutural University,Guangzhou 510642,China);Advances in Purification and Utilization of AChE from Aquatic Animals[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Qiu ju\+1, CHENG Xiao hua\+1, WEI Zhao rong\+1, MA Jie\+2, TIAN Ying\+2, WU Bing\+2, SHAO Jia xing\+3, LIU Biao\+1 (1.Xinjiang Sanitation and Antiepidemic Station, Urumqi Xinjiang 830011, China; 2.Antiepidemic Station of Urumqi Railway Bureau, U;Investigation of Agricultural Chemical Pollution Level in Xinjiang Underground Water[J];Arid Environmental Monitoring;2001-03
2 Cheng jin-lian (The Forest Technical Secondary School of Inner Monogo);THE POLLUTION TO THE ENVIROMEMT AND THE INFLUENCE TO HUMANS HEALTH OF AGRICHEMICALS[J];JOURNAL OF ARID LAND RESOURCES AND ENVIRONMENT;1999-S1
3 WANG Gai-zhen, WEI Fu-xiang, WANG Chang-shi,WANG Xiao-hui, LIANG Gui-yong (College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang Hebei 050018,China;Hebei Qinghe Middle School,Qinghe Hebei 054800,China);Development of enzyme biosensor of organophosphorus pesticides[J];Hebei Journal of Industrial Science & Technaology;2005-04
4 Jiang Xi-liu(Nanjing Institute of Environmental Sciences,National Environmental Protection Agency of China.Nanjing 210042);STUDY ON SCREENING OF THE PRIORITY MONITORING PESTICIDES[J];;1993-06
5 Hua Xiaomei Shan Zhengjun (Nanjing Institute of Environmental Sciences,NEPA,Nanjing 210042);THE PRODUCTICN AND APPLICATION OF PESTIDICES AND FACTOR ANALYSIS OF THEIR POLLUTION IN ENVIRONMENT IN CHINA[J];Advances in Environmental Science;1996-02
6 ZHU Xiao-shan~1,MENG Fan-ping~2,ZHU Lin~1,HE Dong-hai~2,YANG Yao~1(1.College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China;2.College of Marine Life Science,Ocean University of China,Qingdao 266003,China);Selection Study on the Sensitivity of Marine Fishes Brain Acetylcholinesterase to Organophosphorus Pesticides[J];Environmental Science;2006-03
7 XU WeiLianyungang Sanitation and Anti-epidemic Station of Jiangsu Province,222003;Determination of Residual Amount of 8 Kinds of Pesticide in Environmental Water by High Performance Liquid Chromatography[J];Journal of Environment and Health;2000-06
8 Li Hongli Sun Zongguang, Gong Zhengyu, Li Guogang(Shandong Province Environmental Monitoring Center, Jinan 250013) (China National Environmental Monitoring Center, Beijing 100029);DETERMINATION OF PHOSPHORUS-CONTAINING PESTICIDES IN ENVIRONMENTAL WATER SAMPLE BY GAS CHROMATOGRAPHY WITH A FLAME PHOTOMETRIC DETECTOR[J];Chemical Analysis and Meterage;2001-01
9 DING Hongxiang,YU Jing,HU Yunliang,et al.Department of Cli nical Labo-ratory,The Second Affiliated Hospital,Wenzhou Medical Collage Wenzhou325027,Chi na;Rapid determination of organophosphates in human urinem by colorimetric method.[J];Jiangxi Journal of Medical Laboratory Sciences;2003-04
10 ZHANG Ying~(1, 2),HUANG Jian~(1),GAO Xi_Wu~(2*)?(1. College of Plant Protection,Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2. Department of Entomology, China Agricultural University, Beijing 100094, China);The optimization of conditions for assaying activity of acetylcholinesterase from the Italian honeybee (Apis mellifera ligustica Spinola) by orthogonal matrix method[J];Acta Entomologica Sinica;2005-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved