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《Environmental Science & Technology》 2013-07
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Isolation and Identification of an Erythromycin Degradation Bacterium and Study on Its Biodegradation Characteristics

MAO Fei-fei,LIU Chang,HE Meng-qi,SHI Wei-min,XUE Gang*,GAO Pin*(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China)  
Antibiotics have been frequently detected in environmental media and their transport behavior and contamination impacts are attracted worldwide attentions.A strain of erythromycin-degrading bacteria was isolated from activated sludge and denoted as Ery-E.Based on analysis of bacterial morphology,physiological-biochemical characteristics and 16S rDNA sequence,Ery-E was identified as Pseudomonas putida.The study demonstrated that Ery-E could survive using erythromycin as the sole carbon source.The optimum reaction conditions of 30℃,pH 7.0 ~7.5,initial concentration of erythromycin 30 mg/L would lead to erythromycin degradation rate for 5d as high as 76.6%.The biodegradation mechanism of erythromycin was confirmed as co-metabolism.Results indicated that the addition of extra carbon sources could enhance the biodegradation of erythromycin,which reached as high as 83.9% for 5d as the yeast powder was added at 10mg/L.
【Fund】: 国家自然科学基金项目(51178093;51208086);; 高等学校博士学科点专项科研基金(20090075110007)
【CateGory Index】: X172
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