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《Environmental Science & Technology》 2014-03
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Optimization of Sample Pretreatment for Qualification of the Microbes in Solid State Anaerobic Digestion of Corn Stover by Fluorescence in situ Hybridization

SHEN Ruixia;ZHU Jiying;School of Agricultural and Food Engineering, Shandong University of Technology;  
Sample pretreatment method for qualification of the microbes in dry anaerobic digestion by FISH was optimized. Results showed that using absolute ethanol as cleaning fluid, area and abundance of fluorescence signal of the FISH sample was significantly improved than that of PBS. Adding sterilized glass beads in the process of sample stirring can improve the dispersion of microbe cells and then enhance the accuracy of the microbial quantification by the FISH technical for dry anaerobic digestion. When centrifuging with 13 000 r/min for 3 min, the conversion rate is increased, and it will ensure the integrity of cell morphology and lay the foundation for the experiment of subsequent FISH hybridization.
【Fund】: 国家自然科学基金(51008185);; 山东省中青年科学家科研奖励基金(BS2011NJ015);; 山东理工大学青年支持计划(110011)
【CateGory Index】: X172
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