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《Journal of Fujian Forestry Science and Technology》 2007-04
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Optimization of SRAP-PCR in Dimocarpus longan Lour.

JIANG Fan1,2,GAO Hui-ying1,3,CHEN Xiu-ping1,2 ZHENG Shao-quan1,2(1.Fujian Fruit Tree Breeding Engineering Technology Research Center for Longan and Loquat,Fuzhou,Fujian 350013,China;2.Fruit Tree Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;3.Agricultural College,Hebei Engineering University,Handan,Hebei 056038,China)  
The concentrations of primers,dNTP, DNA,Mg2+ and Tm which affect the SRAP-PCR reactions were optimized in order to establish the SRAP molecular marker system in Dimocarpus longan Lour.The optimum system was as follows: 10×buffer(Mg free) 2.5 μl,dNTP mixture(10 mmol·L-1) 2.0 μl,primer(10 μmol·L-1):(0.7+0.7) μl,Mg2+(25 mmol/L) 1.0 μl,DNA 40 ng,Taq DNA polymerase 1.5 U,Tm 51℃. Total volume of reaction was 25 μl.Amplifications were carried out on 16 genotypes of D.longan from China,Thailand and Indonesia using this optimum system.The results showed that the system was steady and reliable.
【Fund】: 福建省农业科技重大专项(2004NE02-1);; 省财政专项—福建省农业科学院创新团队建设基金资助(STIF-Y06)
【CateGory Index】: S667.2
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