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《Journal of Fujian Forestry Science and Technology》 2009-02
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Preliminary Study on the Protoplasts Isolation of Manilkara zapota

LUO Li-hua,YANG Ding-hai,LIN You-he,WANG Yehua(Horticulture and Garden College of Hainan University,Haikou 570228,Hainan,China)  
The tender leaves of Manilkara zapota were used as experiment materials and the influences of enzyme solution combinations,zymohydrolysis time and mannitol concentration in the enzyme solution,pH value,and temperature on protoplast isolation were studied.The results showed that the highest yield(12.4×106/g) was obtained by treating the leaves with a mixture of 3% of cellulose enzyme R-10+0.4% of pectase Y-23 with pH value of 5.6 for 2h of zymohydrolysis time at the temperature of 28℃,and on the oscillation condition with 0.6 mol·L-1 of mannitol as the osmotic pressure stabilizer.
【CateGory Index】: S667.9
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