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《Journal of Fujian Agriculture and Forestry University(Natural Science Edition)》 1993-04
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Culture of the Immature Embryo of Litchi in Vitro

Lu Liuxin;Yu Xiaoling Dept of Horticulture,FAU,Fuzhou 350002  
Immature embryos of 3 litchi cultivars(Wuye,Lanzhu and Luhebao,belonging to 3 types of embryonic development,respectively)were cultured on MS media supplemented with growth regulators.The results showed that it is very difficulty to culture litchi immature embryos 15 d after pollination.But most of embryos 30-40 d after pollination could be induced into cell aggregate on MS media containing BA,NAA, GA and ABA,and further develop into embryoids and bud-like bodies.Embryoids were also induced from the immature embryos of partly abortive cv.Lanzhu in the early period of abortion but not being died,and ovules picked up 30 d after pollination from completely abortive cv.Luhebao.The multiplication capability of embryoids was very strong when they were cultured on MS or 1/2 MS media supplemented with the low level of cytokinin and other growth regulators,even multiplicated on the MS media without any growth regu- lator.High level supplements inhibited the multiplication of embryoids.Further development of embryoids into plantlets should be further studied.
【CateGory Index】: S667.1
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